The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
| Technical specifications | |||||||
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. | ||||||
| Elution Volume | ≥ 50 µl DNA Elution Buffer or DNase free water | ||||||
| Equipment | Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended). | ||||||
| Processing Volume | Fecal ≤200 mg, Soil ≤ 250 mg, Cells ≤ 100 mg (Approximately equal to 2×109bacterial, 2×108yeast, or 2×107mammalian cells) | ||||||
| Purity | High quality, inhibitor-free DNA. Typical A260/A280 ≥1.8 | ||||||
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms, and water. | ||||||
| Sample Storage | Eluted DNA should be stored at ≤ -20°C | ||||||
| Size Range | Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. | ||||||
| Type | Total DNA | ||||||
| Yield | Up to 25 µg total DNA can be eluted into ≥50 µl | ||||||
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
A-104405, 3-Part Life-Size Human Skull Axis Scientific
QIAamp DNA Blood Mini Kit (250) - 51106 - Qiagen
A-105939, 22 Part Osteopathic Didactic Human Skull Axis Scientific
A-104406 Complete Disarticulated Human Skeleton Axis Scientific
V1002-S8 Optional eight channel adapter, fine tip stainless steel Accuris
A-106193, Didactic Skull On Cervical Vertebrae With Nerves And Arteries Axis Scientific
A-105852 Classic Flexible Human Skeleton Axis Scientific
A-105177 Painted And Numbered 3-Part Life-Size Human Skull Axis Scientific
A-104281 Painted Human Skeleton Axis Scientific
A-105168 Miniature Human Skeleton Axis Scientific
0090 Natural Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
0096F WELL MICROCENTRIFUGE TUBE RACK, FLUORESCENT YELLOW, 5/PK Bio Plas
A-105170 Miniature Painted Human Skeleton Axis Scientific
A-104272 Life-Size Human Skull With Blood Vessels Axis Scientific
A-105868, Human Skull With Masticatory Muscles Axis Scientific
R1056- Quick-RNA Midiprep Kit 25 preps- Zymo Research
A-105866 Miniature Skeleton With Flexible Spine Axis Scientific
A-105867, Human Skull Model With Flexible Neck Axis Scientific
0093 Green Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-105171 Half Disarticulated Human Skeleton Axis Scientific
EC-5108A Evatros C(200ml Tube, 8 Hole) + Evatros G (200L/min Dry Air) Goojung
850071 Environmental Quality SD Card Logger Sper Scientific
A-104270, 3-Part Life-Size Numbered Human Skull Axis Scientific
A-104264, 16-Part Mini Torso Axis Scientific
A-105176 Disarticulated Half Child Skeleton Axis Scientific
V1002 Handheld vacuum controller with single channel adapters Accuris
A-104271, 3-Part Life-Size Painted Didactic Human Skull Axis Scientific
D4075- Quick-DNA Midiprep Plus Kit- Zymo Research 



