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R2062/R2061/R2063/R2060- Direct-zol RNA Microprep Kits- Zymo Research


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The Direct-zol RNA MicroPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 10 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol™ method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin™ Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.). The entire procedure typically takes only 7 minutes.



Technical specifications
Compatibility TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®.
Equipment Microcentrifuge, vortex
Sample Inactivation TRI Reagent® (provided with R2061, R2063) inhibits RNase activity and inactivates viruses and other infectious agents.
Sample Source Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).
Size Range Total RNA ≥ 17 nt
Yield 10 µg RNA (binding capacity), ≥6 µl (elution volume)
SKU: R2062/R2061/R2063/R2060 Categories: , , Tags: , , , Brand:
Product

R2062 (Direct-zol RNA Microprep – 200 preps), R2061 (Direct-zol RNA Microprep (Product Supplied w/ 50 ml TRI Reagent) – 50 preps), R2063 (Direct-zol RNA Microprep (Product Supplied w/ 200 ml TRI Reagent) – 200 preps), R2060 (Direct-zol RNA Microprep – 50 preps)

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