The Mix & Go! E. coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation. Mix & Go! E. coli cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − Mix & Go! The premade Mix & Go! competent cells are highly efficient (> 108 transformants/µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.
| Technical specifications | |||||||
| Additional Info | Can be used for blue/white screening and is ideal for cDNA generation and library construction. | ||||||
| Genotype | F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara leu) 7697 galU galK rpsL nupG λ- | ||||||
| Processing Time | 20 Seconds | ||||||
| Product Storage | -70°C to -80°C | ||||||
| Transformation Efficiency | 108 – 109 transformants per µg of plasmid DNA | ||||||
D4075- Quick-DNA Midiprep Plus Kit- Zymo Research
QIAamp DNA Blood Mini Kit (250) - 51106 - Qiagen
A-105170 Miniature Painted Human Skeleton Axis Scientific
V0020-F1 Hydrophobic filter, pk/5 ( 1 each included) Accuris
A-105173 Miniature Skull Axis Scientific
RE301B-OV - Rotary evaporator, Glass "B", Oil Bath, VR300RE301B-OV - Yamato
D4074- Quick-DNA Microprep Plus Kit- Zymo Research
A-104270, 3-Part Life-Size Numbered Human Skull Axis Scientific
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
A-105852 Classic Flexible Human Skeleton Axis Scientific
A-105939, 22 Part Osteopathic Didactic Human Skull Axis Scientific
BSW1516 Block For Digital Dry Bath - 12 x 15-16mm or 12 x 10ml Tubes Benchmark Scientific (Copy)
0097 Lavender Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
840003 Digital Anemometer Thermometer Sper Scientific
RC 900 - Rotary Evaporator- Logos BioSystem
28506-QIAquick Spin Columns (100)- Qiagen
A-105177 Painted And Numbered 3-Part Life-Size Human Skull Axis Scientific
A-105868, Human Skull With Masticatory Muscles Axis Scientific
BSWMT Block, 1 x Micro Plate (2 & 4 block models only) Benchmark Scientific
0090 Natural Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-104405, 3-Part Life-Size Human Skull Axis Scientific
0095 Pink Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
V1002-8 Optional eight channel adapter, for use with 200ul pipette tips Accuris 


