The Mix & Go! E. coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation. Mix & Go! E. coli cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − Mix & Go! The premade Mix & Go! competent cells are highly efficient (> 108 transformants/µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.
| Technical specifications | |||||||
| Additional Info | Can be used for blue/white screening and is ideal for cDNA generation and library construction. | ||||||
| Genotype | F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara leu) 7697 galU galK rpsL nupG λ- | ||||||
| Processing Time | 20 Seconds | ||||||
| Product Storage | -70°C to -80°C | ||||||
| Transformation Efficiency | 108 – 109 transformants per µg of plasmid DNA | ||||||
R1056- Quick-RNA Midiprep Kit 25 preps- Zymo Research
D4075- Quick-DNA Midiprep Plus Kit- Zymo Research
0094 Orange Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-105759, Life-Size Human Skull With Removable Muscles Axis Scientific
3970422- 4' Purifier Filtered PCR Enclosure with UV Light and Protection Panel- Labconco
A-105868, Human Skull With Masticatory Muscles Axis Scientific
701811230-FreeZone 18 Liter -50C Console Freeze Dryer- Labconco
A-105176 Disarticulated Half Child Skeleton Axis Scientific
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
0092F Fluorescent Blue Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-106193, Didactic Skull On Cervical Vertebrae With Nerves And Arteries Axis Scientific
A-104272 Life-Size Human Skull With Blood Vessels Axis Scientific
700612030- FreeZone 6 Liter -50C Console Freeze Dryer- Labconco
700402030- FreeZone 4.5 Liter -50C Benchtop Freeze Dryer- Labconco
710401000-FreeZone 4.5 Liter -84C Benchtop Freeze Dryer- Labconco
D4204 - ZymoPURE II Plasmid Gigaprep Kit 5 Preps- Zymo Research
20X Tris Buffered Saline with Tween 20, pH 7.4 - Hoefer
A-105178, 3-Part Life-Size Human Skull With 8-Part Brain Axis Scientific
A-105179, 22-Part Disarticulated Life-Size Human Skull Axis Scientific
A-105171 Half Disarticulated Human Skeleton Axis Scientific
D4074- Quick-DNA Microprep Plus Kit- Zymo Research
700201030- FreeZone 2.5 Liter -50C Benchtop Freeze Dryer- Labconco
QIAamp DNA Blood Mini Kit (250) - 51106 - Qiagen
A-105173 Miniature Skull Axis Scientific 


