This assay has high sensitivity and excellent specificity for detecting Mouse Alanine Aminotransferase (ALT). The assay range is from 1.56 to 100 ng/ml (Sandwich kit) with a sensitivity of 0.61 ng/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.
- High sensitivity and specificity
- Perfect reproducibility and consistency across batches
- Quality control with three-level inspections
- Wide range of targets/species available
- Intra-assay: CV<10%; Inter-assay: CV<12%
Alanine transaminase or ALT is a transaminase enzyme. It is also called serum glutamic pyruvic transaminase (SGPT) or alanine aminotransferase (ALAT). ALT is found in serum and in various bodily tissues, but is most commonly associated with the liver. It catalyzes the transfer of an amino group from alanine to a-ketoglutarate, the products of this reversible transamination reaction being pyruvate and glutamate. It is commonly measured clinically as a part of a diagnostic liver function test, to determine liver health. GPT1 transcript is moderately expressed in kidney, liver, heart, and fat. Another GPT1 transcript shows a slightly different expression pattern, with a 3.9-kb transcript expressed at high levels in muscle, fat, kidney, and brain and at lower levels in liver and breast.
| Specifications | |||
| Assay duration | Multiple steps | ||
| Assay Type | Sandwich | ||
| Conjugate ELISA | Biotin | ||
| Format | Pre-coated | ||
| Host | Rabbit | ||
| Primary antibody reactivity | Mouse | ||
| Target protein | ALT | ||
| Size | 1 kit | ||
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids | ||
| Cross Reactivity | No significant cross-reactivity or interference between Lipoprotein lipase (LPL) and analogues was observed | ||
| Detection Method | Colorimetric | ||
| Time to Results | 3 h | ||
| Shelf Life | 12 Months | ||
| Detection Range | 1.56 – 100 ng/ml | ||
| Storage Temperature | 4 °C for one month (frequent use), −20 °C for one year | ||
| Sample Volume | 100 µl | ||
| Sensitivity | 0.61 ng/ml | ||
| Regulatory Status | RUO | ||
| No. of tests | 96 wells | ||
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