The ZymoBIOMICS MagBead RNA kit provides a high-throughput, magnetic beadbased purification of total RNA (including small/microRNAs) from any sample source (e.g., microbes including gram positive/negative bacteria, fungi, protozoans, viruses, feces, soil, plant, water, biofilms, swabs, saliva, body fluids and etc.). The provided DNA/RNA Shield inactivates infectious agents and is ideal for sample storage at ambient temperatures. Total RNA is eluted into ≥50 µl of ZymoBIOMICS DNase/RNase-Free Water and is ready for any downstream application including Next-Gen Sequencing, RT-qPCR, etc.
| Technical specifications |
| Binding Capacity |
15 µg total RNA per 30 µl MagBinding Beads. |
| Equipment Needed |
Magnetic stand, heat block, liquid handler or robotic sample processor (user provided). |
| Purity |
High-quality RNA is ready for Next-Gen Sequencing, RT/PCR, hybridization, etc. |
| Recommended Materials |
(sold separately) – 96-well Collection Plate (C2002; capacity is up to 1.2 ml/well), 96-Well Block (P1001; capacity is up to 2 ml/well), 96-well Elution Plate (C2003), Cover Foil (C2007), ZR-96 MagStand (P1005). |
| Sample Preservation |
DNA/RNA Shield lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures. |
| Sample Types |
Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA and RNA are efficiently isolated from ≤ 50mg of mammalian feces, ≤ 50mg soil, and 5-20mg (wet weight) of fungal bacterial cells, biofilms, water, and swabs. |
| Size Limits |
Total RNA including small/microRNAs ≥ 17nt. |
| Storage |
RNA eluted with DNase/RNase-Free Water (provided) can be stored frozen. The addition of RNase inhibitors is highly recommended for prolonged storage |
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