The ZymoBIOMICS RNA Miniprep Kit is designed for purifying RNA from a wide array of sample inputs that is ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae). The procedure uses Zymo-Spin Column technology that results in high-quality total RNA (including small RNAs 17-200 nt) that is free of PCR inhibitors and is ready for RT-PCR, hybridization, sequencing, etc. DNase I included.
| Technical specifications | |||||||
| Equipment | Microcentrifuge, vortex, cell disrupter (recommended) | ||||||
| Purity | RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8. | ||||||
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host RNA is efficiently isolated from ≤ 200 mg of mammalian feces, ≤ 250 mg soil, ≤ 200 mg plant/seed, 50-100 mg (wet weight) fungal bacterial cells, biofilms, and water. | ||||||
| Size Range | Total RNA ≥17 nt | ||||||
| Yield | 100 µg RNA (binding capacity), ≥50 µl (elution volume) | ||||||
PAA643HU01 - Anti-GSTm4 Rabbit Polyclonal Antibody -Cloud-Clone
SPB 723i -Solis Precision Balances- Adam Equipment
LAB 214e -Luna Analytical Balances- Adam Equipment
S66 Premier Flexible Series Skeleton - Sacral Mount, Male- Denoyer Geppert
SEB785HU-Human CD10 ELISA Kit- Cloude Clone
BCLO2025 Protective Cover For Stainless Steel Grid Alliance Bio Expertise
VE-M5DTH DOUBLE HEAD MONOCULAR MICROSCOPE- VELAB
PAS085HU01-Anti-Ang1-7 Rabbit Polyclonal Antibody- Cloude Clone
F144070-PIPETMAN G Multichannel P8x20G, 2-20µL-Gilson
GR149-1 -Tris-Glycin e-SDS Buffer, 10X Solution (Ultrapure) 1LT- Hoefer
FA10015-PIPETMAN L Multichannel P8x300L, 20-300 µL-Gilson
A-105868, Human Skull With Masticatory Muscles Axis Scientific
D5220-1- Micrococcal Nuclease - Zymo Research
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
QIAamp DNA Blood Mini Kit (250) - 51106 - Qiagen
4305 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Amber Bio Plas
0094 Orange Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
D4075- Quick-DNA Midiprep Plus Kit- Zymo Research
D4074- Quick-DNA Microprep Plus Kit- Zymo Research 



