The Quick-RNA Viral 96 Kit is a viral RNA purification kit that provides for rapid, large scale isolation of viral RNA from plasma, serum, cell culture media, cellular suspensions, urine, blood, saliva and any other biological samples stored in DNA/RNA Shield. DNA/RNA Shield ensures nucleic acid stability during sample storage/transport at ambient temperatures. The reagent effectively lyses cells and inactivates nucleases and infectious agents (virus). The kit features a specialized buffer system that facilitates complete viral particle lysis for efficient RNA isolation from samples containing enteroviruses, rhinoviruses, coronaviruses, HIV, HCV, influenza A virus, flaviviruses, measles virus, parainfluenza virus, parvovirus (a ssDNA virus), etc. Viral RNA is bound to the matrix in each well of the spin-plate, washed and eluted. The isolated high-quality viral RNA is ready for all downstream applications such as Next-Gen sequencing, hybridization-based and RT/PCR detection.
| Technical specifications | |||||||
| Equipment | Centrifuge with 96-well plate carrier | ||||||
| Purity | RNA is ready for Next-Gen sequencing, RT-qPCR, microarray, hybridization, etc. | ||||||
| Sample Source | Plasma, serum, saliva, urine, blood, cell culture media, cellular suspensions, biopsies, swab and fecal samples | ||||||
| Size Range | 50 nt to ~200 kb | ||||||
| Yield | Spin plate, 10 µg RNA (binding capacity), ≥10 µl (elution volume) | ||||||
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