The Quick-DNA/RNA™ FFPE Kits provide a simple and reliable method for total nucleic acid isolation (DNA & RNA) from the same formalin-fixed, paraffin embedded (FFPE) tissue sample. The unique chemistries of the product have been optimized for maximum recovery of DNA and of both large and small RNA species. Simply deparaffinize tissues using Deparaffinization Solution, digest using Proteinase K, heat to reverse chemical crosslinks, and then purify using Zymo-Spin™ column technology. The result is high-quality DNA & total RNA (including small RNAs 17-200 nt) that is DNA-free and is ready for RT-PCR, hybridization, sequencing, etc.
| Technical specifications | |||||||
| Equipment | Microcentrifuge, vortex, and heat block/bath | ||||||
| Purity | DNA/RNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: ≥1.8. | ||||||
| Sample Source | Up to 25 mg tissue from paraffin block or up to four (4) tissue sections (≤20 µm thick) with a total surface area ~20 mm2 | ||||||
| Size Range | DNA and Total RNA ≥17 nt | ||||||
| Yield | 50 µg DNA/RNA (binding capacity), ≥25 µl (elution volume) | ||||||
QIAamp DNA Blood Mini Kit (250) - 51106 - Qiagen
0096F WELL MICROCENTRIFUGE TUBE RACK, FLUORESCENT YELLOW, 5/PK Bio Plas
A-105177 Painted And Numbered 3-Part Life-Size Human Skull Axis Scientific
A-105866 Miniature Skeleton With Flexible Spine Axis Scientific
V1002 Handheld vacuum controller with single channel adapters Accuris
A-105179, 22-Part Disarticulated Life-Size Human Skull Axis Scientific
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
A-105173 Miniature Skull Axis Scientific
A-105171 Half Disarticulated Human Skeleton Axis Scientific
A-105759, Life-Size Human Skull With Removable Muscles Axis Scientific
A-104267 Classic Human Skeleton With Study & Numbering Guide Axis Scientific
0052 Snap On Tube Rack Cover, 96-Well, Polypropylene, Clear Natural Bio Plas
R1056- Quick-RNA Midiprep Kit 25 preps- Zymo Research 


