MX027 – TGEX-HC-hG2-Zeo – Human IgG2 Mammalian Expression Vector -Antibody Design

Technical Description

The TGEX™ vector series is designed for the rapid expression of antibody molecules by transient gene expression in mammalian cell suspension cultures. TGEX™-HC-hG2-Zeo expresses the constant region of the human IgG2 heavy chain. Expression of full length antibody molecules is achieved by co-transfection with a light chain variable region cloned into one of the TGEX™-LC vector series. Using transient transfection technologies yields of antibody between 20 mg/L and 200 mg/L and more in serum-free conditions can be obtained in just a few days.

Applications

• Mammalian expression of N-terminal Fc fusion molecules by transient gene expression.

• Selection of stable transfectants using Zeocin™.

Specifications

General Characteristics:

Plasmid Size: 5816
Promoter: CMV + TPL
Leader Peptide: pelBK
Cloning Site: Double BsaI
Fusion Protein: full length IgG2 human constant region
Selection: ampicillin (bacteria), zeocin™ (mammalian)
Replication: pMB1 high copy number

Physical Characteristics:

Concentration: 0.5 µg/µl.
Product Size: 10 µg.
Buffer: DNA Conservation Buffer (Tris/HCL 5 mM, EDTA 0.1 mM, pH 8.5, sterile).
Storage Temperature: -20°C.