ZymoTaq DNA Polymerase contains all the reagents needed to perform “hot-start” PCR. The inclusion of a heat-activated, thermostable DNA polymerase reduces primer dimer and nonspecific product formation that can occur during PCR. This unique product is specifically designed for the amplification of bisulfite-treated DNA for methylation detection, but is applicable for conventional PCR. The product generates specific amplicons with little or no by-product formation. Simple and easy to use: Heat at 95°C for 10 minutes to initiate polymerization. ZymoTaq DNA Polymerase is a heat-activated, “hot start” polymerase that has 3′-terminal transferase activity. The addition of “A” overhangs to amplified DNA makes it ideal for use in TA-cloning.
| Technical specifications | |||||||
| Heat Inactivation | 95°C for 10 minutes | ||||||
| Storage | -20°C | ||||||
| Templates | DNA, bisulfite-converted DNA, CpG rich DNA | ||||||
| Product | E2001(ZymoTaq DNA Polymerase – 50 Rxns), E2002 (ZymoTaq DNA Polymerase – 200 Rxns) |
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