The Methylated-DNA IP Kit is designed for enrichment of 5mC-containing DNA from any pool of fragmented genomic DNA for use in genome-wide methylation analysis. It features a highly specific Anti-5-Methylcytosine Monoclonal Antibody for the immunoprecipitation of methylated DNA in only a few hours. This kit is capable of achieving over one hundred-fold enrichment of methylated DNA vs. non-methylated DNA. Recovered DNA is suitable for many downstream applications to analyze genome-wide DNA methylation including PCR, bisulfite treatment, whole-genome amplification, ultra-deep sequencing, and microarray. Control DNA and primers are included to monitor the success of the assay.
| Technical specifications | |||||||
| Applicable For | Immunoprecipitation of methylated DNA, PCR, sequencing, microarrays, etc. | ||||||
| Elution Volume | ≥ 15 µl | ||||||
| Input | 50 – 500 ng of DNA | ||||||
| Processing Time | 4 hours | ||||||
GR153-1 -Boric Acid, ACS Reagent Grade 1KG- Hoefer
A-104270, 3-Part Life-Size Numbered Human Skull Axis Scientific
RE301A-WV - Rotary evaporator, Glass "A", Water Bath, VR300- Yamato
0093F Fluorescent Green Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
0094 Orange Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-105866 Miniature Skeleton With Flexible Spine Axis Scientific
BSW1520 Block For Digital Dry Bath - 24 x 1.5ml or 2ml Tubes Benchmark Scientific
D4074- Quick-DNA Microprep Plus Kit- Zymo Research
0091F Assorted Fluorescent Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-105171 Half Disarticulated Human Skeleton Axis Scientific
RE801C-W- Rotary Evaporator - Yamato
0095F Fluorescent Pink Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
0092 Blue Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-104267 Classic Human Skeleton With Study & Numbering Guide Axis Scientific
A-105759, Life-Size Human Skull With Removable Muscles Axis Scientific
0090 Natural Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-104406 Complete Disarticulated Human Skeleton Axis Scientific
D4075- Quick-DNA Midiprep Plus Kit- Zymo Research 


