The Methylated-DNA IP Kit is designed for enrichment of 5mC-containing DNA from any pool of fragmented genomic DNA for use in genome-wide methylation analysis. It features a highly specific Anti-5-Methylcytosine Monoclonal Antibody for the immunoprecipitation of methylated DNA in only a few hours. This kit is capable of achieving over one hundred-fold enrichment of methylated DNA vs. non-methylated DNA. Recovered DNA is suitable for many downstream applications to analyze genome-wide DNA methylation including PCR, bisulfite treatment, whole-genome amplification, ultra-deep sequencing, and microarray. Control DNA and primers are included to monitor the success of the assay.
| Technical specifications | |||||||
| Applicable For | Immunoprecipitation of methylated DNA, PCR, sequencing, microarrays, etc. | ||||||
| Elution Volume | ≥ 15 µl | ||||||
| Input | 50 – 500 ng of DNA | ||||||
| Processing Time | 4 hours | ||||||
4303 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Blue Bio Plas
4301 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Red Bio Plas
0095 Pink Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-104267 Classic Human Skeleton With Study & Numbering Guide Axis Scientific
D4075- Quick-DNA Midiprep Plus Kit- Zymo Research
0091F Assorted Fluorescent Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
D4074- Quick-DNA Microprep Plus Kit- Zymo Research
A-105178, 3-Part Life-Size Human Skull With 8-Part Brain Axis Scientific
0096 Well Microcentrifuge Tube Rack, Yellow, 5/Pk
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
A-105866 Miniature Skeleton With Flexible Spine Axis Scientific
R1056- Quick-RNA Midiprep Kit 25 preps- Zymo Research
QIAamp DNA Blood Mini Kit (250) - 51106 - Qiagen 

