Kits for Epigenetics, Reagent and Kits
D5021/D5020 – EZ DNA Methylation-Direct Kit- Zymo Research
$213.00 – $602.00
The EZ DNA Methylation-Direct kit is a bisulfite conversion kit that features reliable and complete DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of this bisulfite conversion kit makes it possible to amplify bisulfite-converted DNA from as few as 10 cells or 50 pg DNA. Recovered bisulfite-converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.
| Technical specifications | |||||||
| Applications | Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc. | ||||||
| Conversion | > 99.5% | ||||||
| Elution Volume | ≥ 10 µl | ||||||
| Equipment | Thermocycler with heated lid and microcentrifuge. | ||||||
| Input | Samples containing between 50 pg to 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng. The number of cells per standard treatment can range from 10-105 cells. | ||||||
| Processing Time | 4 hours | ||||||
| Recovery | > 80% | ||||||
| Sample Source | Blood, tissue, cells, FFPE, LCM-derived samples, purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. | ||||||
| Product | D5021 (EZ DNA Methylation-Direct Kit – 200 Rxns), D5020 (EZ DNA Methylation-Direct Kit – 50 Rxns.) |
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