The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
| Technical specifications | |||||||
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing | ||||||
| Elution Volume | ≥ 20 µl DNA Elution Buffer or DNase free water | ||||||
| Equipment | Centrifuge w/ microplate carriers, 96-well plate/block disruptor or pulverizer | ||||||
| Processing Volume | Fecal ≤100 mg, Soil ≤ 100 mg, Cells ≤ 20 mg (Approximately equal to 2×108bacterial, 2×107yeast, or 2×106mammalian cells) | ||||||
| Purity | High quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing | ||||||
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. | ||||||
| Sample Storage | Eluted DNA should be stored at ≤ -20°C. | ||||||
| Size Range | Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. | ||||||
| Type | Total DNA | ||||||
| Yield | Up to 5 µg total DNA can be eluted into 20 µl | ||||||
0099 Black Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
0097 Lavender Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
0094 Orange Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
0093F Fluorescent Green Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
0092 Blue Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-105173 Miniature Skull Axis Scientific
A-105170 Miniature Painted Human Skeleton Axis Scientific
0096 Well Microcentrifuge Tube Rack, Yellow, 5/Pk
A-105179, 22-Part Disarticulated Life-Size Human Skull Axis Scientific
A-104270, 3-Part Life-Size Numbered Human Skull Axis Scientific
4301 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Red Bio Plas
A-105177 Painted And Numbered 3-Part Life-Size Human Skull Axis Scientific
A-105178, 3-Part Life-Size Human Skull With 8-Part Brain Axis Scientific
A-104405, 3-Part Life-Size Human Skull Axis Scientific
R1056- Quick-RNA Midiprep Kit 25 preps- Zymo Research
A-104272 Life-Size Human Skull With Blood Vessels Axis Scientific
4300 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Natural Bio Plas
QIAamp DNA Blood Mini Kit (250) - 51106 - Qiagen
A-105940, 22 Part Osteopathic Natural Bone Human Skull Axis Scientific
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
D4075- Quick-DNA Midiprep Plus Kit- Zymo Research
4307 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Lavender Bio Plas
A-104406 Complete Disarticulated Human Skeleton Axis Scientific
A-105171 Half Disarticulated Human Skeleton Axis Scientific
D4074- Quick-DNA Microprep Plus Kit- Zymo Research
A-105759, Life-Size Human Skull With Removable Muscles Axis Scientific
A-105868, Human Skull With Masticatory Muscles Axis Scientific
A-104267 Classic Human Skeleton With Study & Numbering Guide Axis Scientific
A-105852 Classic Flexible Human Skeleton Axis Scientific
A-106193, Didactic Skull On Cervical Vertebrae With Nerves And Arteries Axis Scientific
RE601B-W - Rotary EvaporatorRE601B-W - Yamato
0095 Pink Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-104271, 3-Part Life-Size Painted Didactic Human Skull Axis Scientific
A-105866 Miniature Skeleton With Flexible Spine Axis Scientific 

