The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
| Technical specifications | |||||||
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing | ||||||
| Elution Volume | ≥ 20 µl DNA Elution Buffer or DNase free water | ||||||
| Equipment | Centrifuge w/ microplate carriers, 96-well plate/block disruptor or pulverizer | ||||||
| Processing Volume | Fecal ≤100 mg, Soil ≤ 100 mg, Cells ≤ 20 mg (Approximately equal to 2×108bacterial, 2×107yeast, or 2×106mammalian cells) | ||||||
| Purity | High quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing | ||||||
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. | ||||||
| Sample Storage | Eluted DNA should be stored at ≤ -20°C. | ||||||
| Size Range | Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. | ||||||
| Type | Total DNA | ||||||
| Yield | Up to 5 µg total DNA can be eluted into 20 µl | ||||||
3970303- 3' Purifier Vertical Clean Bench with Airflow Monitor- Labconco
711221010-FreeZone 12 Liter -84C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
A-105179, 22-Part Disarticulated Life-Size Human Skull Axis Scientific
A-105170 Miniature Painted Human Skeleton Axis Scientific
A-105940, 22 Part Osteopathic Natural Bone Human Skull Axis Scientific
D4075- Quick-DNA Midiprep Plus Kit- Zymo Research
0097 Lavender Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-105177 Painted And Numbered 3-Part Life-Size Human Skull Axis Scientific
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
794801210-Stoppering Tray Dryer- Labconco
28506-QIAquick Spin Columns (100)- Qiagen
0090 Natural Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-105759, Life-Size Human Skull With Removable Muscles Axis Scientific
RE301C-WV - Rotary evaporator, Glass "C", Water Bath, VR300 - Yamato
0091F Assorted Fluorescent Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
WashLab790 Laboratory Bottle Washer Kimadi
A-105171 Half Disarticulated Human Skeleton Axis Scientific
D4074- Quick-DNA Microprep Plus Kit- Zymo Research
A-105939, 22 Part Osteopathic Didactic Human Skull Axis Scientific
A-105866 Miniature Skeleton With Flexible Spine Axis Scientific
A-104281 Painted Human Skeleton Axis Scientific
3970223- 2' Purifier Vertical Clean Bench with Airflow Monitor- Labconco
0095 Pink Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
3970401- 4' Purifier Vertical Clean Bench with UV Light and Protection Panel- Labconco
R1056- Quick-RNA Midiprep Kit 25 preps- Zymo Research
0093F Fluorescent Green Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
3970320- 3' Purifier Vertical Clean Bench- Labconco
3970222- 2' Purifier Filtered PCR Enclosure with UV Light and Protection Panel- Labconco
700621030- FreeZone 6 Liter -50C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
711222110-FreeZone 12 Liter -84C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
0096 Well Microcentrifuge Tube Rack, Yellow, 5/Pk
QIAamp DNA Blood Mini Kit (250) - 51106 - Qiagen
3970221- 2' Purifier Vertical Clean Bench with UV Light and Protection Panel- Labconco
711212010-FreeZone 12 Liter -84C Console Freeze Dryer- Labconco
780701040-Bulk Tray Dryer- Labconco
0092F Fluorescent Blue Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
711222010-FreeZone 12 Liter -84C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
3970302- 3' Purifier Filtered PCR Enclosure with UV Light and Protection Panel- Labconco 



