The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
| Technical specifications | |||||||
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing | ||||||
| Elution Volume | ≥ 20 µl DNA Elution Buffer or DNase free water | ||||||
| Equipment | Centrifuge w/ microplate carriers, 96-well plate/block disruptor or pulverizer | ||||||
| Processing Volume | Fecal ≤100 mg, Soil ≤ 100 mg, Cells ≤ 20 mg (Approximately equal to 2×108bacterial, 2×107yeast, or 2×106mammalian cells) | ||||||
| Purity | High quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing | ||||||
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. | ||||||
| Sample Storage | Eluted DNA should be stored at ≤ -20°C. | ||||||
| Size Range | Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. | ||||||
| Type | Total DNA | ||||||
| Yield | Up to 5 µg total DNA can be eluted into 20 µl | ||||||
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
SmartReader 96, Microplate Absorbance Reader Accuris
4303 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Blue Bio Plas
0091 Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
4307 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Lavender Bio Plas
4000 Blue Polypropylene Snap Cap Microcentrifuge Tube Opening Tool (Case Of 100) Bio Plas
0092 Blue Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
DR200 Natural Convection Oven Yamato 



