The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
| Technical specifications | |||||||
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. | ||||||
| Elution Volume | ≥ 37.5 µl DNA Elution Buffer or DNase free water | ||||||
| Equipment | Centrifuge fitted with a 96 well microplate carrier, 96 Well Magnetic Stand, Liquid handler or other robotic sample processor, 96 well plate heat block | ||||||
| Processing Volume | Fecal ≤100 mg, Soil ≤ 200 mg, Cells ≤ 20 mg (Approximately equal to 2×108bacterial, 2×107yeast, or 2×106mammalian cells) | ||||||
| Purity | High quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing | ||||||
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. | ||||||
| Sample Storage | Eluted DNA should be stored at ≤ -20°C | ||||||
| Size Range | Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. | ||||||
| Type | Total DNA | ||||||
| Yield | Up to 10 µg total DNA can be eluted into 50 µl (37.5 µl minimum) | ||||||
A-105866 Miniature Skeleton With Flexible Spine Axis Scientific
A-104281 Painted Human Skeleton Axis Scientific
A-104271, 3-Part Life-Size Painted Didactic Human Skull Axis Scientific
SF4500- Stat Fax®4500- Awareness Technology
850071 Environmental Quality SD Card Logger Sper Scientific
A11-000- SelectaLyte™ ISE Analyzer 510(k) Cleared- Awareness Technology
SF4400- LuMate® 4400- Awareness Technology
SF 3300- STAT FAX 3300- Awareness Technology
A-104405, 3-Part Life-Size Human Skull Axis Scientific
SF2200- Stat Fax®2200- Awareness Technology
SF4200- Stat Fax®4200- Awareness Technology
A-104272 Life-Size Human Skull With Blood Vessels Axis Scientific
A-105178, 3-Part Life-Size Human Skull With 8-Part Brain Axis Scientific
0090 Natural Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
0095 Pink Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-105176 Disarticulated Half Child Skeleton Axis Scientific
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
SF2600- Stat Fax®2600- Awareness Technology
WashLab790 Laboratory Bottle Washer Kimadi
A-105168 Miniature Human Skeleton Axis Scientific
SF4700- Stat Fax® 4700- Awareness Technology
V1002-8 Optional eight channel adapter, for use with 200ul pipette tips Accuris
A-104270, 3-Part Life-Size Numbered Human Skull Axis Scientific
SF4300- Stat Fax 4300 (Chromate)- Awareness Technology
A-105170 Miniature Painted Human Skeleton Axis Scientific
840003 Digital Anemometer Thermometer Sper Scientific 



