The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of tough microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
| Technical specifications | |||||||
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. | ||||||
| Elution Volume | ≥ 10 µl ZymoBIOMICS DNase/RNase Free Water | ||||||
| Equipment | Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended). | ||||||
| Processing Volume | Fecal ≤50 mg, Soil ≤ 100 mg, Cells ≤ 20 mg (Approximately equal to 2×108bacterial, 2×107yeast, or 2×106mammalian cells) | ||||||
| Purity | High quality, inhibitor-free DNA. Typical A260/A280 ≥1.8 | ||||||
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. | ||||||
| Sample Storage | Eluted DNA should be stored at ≤ -20°C. | ||||||
| Size Range | Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield | ||||||
| Type | Total DNA | ||||||
| Yield | Up to 5 µg total DNA can be eluted into ≥ 10 µl | ||||||
4305 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Amber Bio Plas
0093 Green Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
QIAamp DNA Blood Mini Kit (250) - 51106 - Qiagen
4300 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Natural Bio Plas
0052 Snap On Tube Rack Cover, 96-Well, Polypropylene, Clear Natural Bio Plas
A-104264, 16-Part Mini Torso Axis Scientific
850071 Environmental Quality SD Card Logger Sper Scientific
A-105173 Miniature Skull Axis Scientific
4303 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Blue Bio Plas
4306 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Orange Bio Plas
Q620 WashLab760 Laboratory Bottle Washer Kimadi
A-104271, 3-Part Life-Size Painted Didactic Human Skull Axis Scientific
A-105172 Human Skeleton With Flexible Spine, Muscle Insertions, And Ligaments Axis Scientific
A-104272 Life-Size Human Skull With Blood Vessels Axis Scientific
A-106193, Didactic Skull On Cervical Vertebrae With Nerves And Arteries Axis Scientific
A-105852 Classic Flexible Human Skeleton Axis Scientific
A-105866 Miniature Skeleton With Flexible Spine Axis Scientific
A-105939, 22 Part Osteopathic Didactic Human Skull Axis Scientific
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
Q720 WashLab770 Laboratory Bottle Washer Kimadi
A-104406 Complete Disarticulated Human Skeleton Axis Scientific
4307 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Lavender Bio Plas
0091 Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
R1056- Quick-RNA Midiprep Kit 25 preps- Zymo Research 



