The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of tough microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
| Technical specifications | |||||||
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. | ||||||
| Elution Volume | ≥ 10 µl ZymoBIOMICS DNase/RNase Free Water | ||||||
| Equipment | Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended). | ||||||
| Processing Volume | Fecal ≤50 mg, Soil ≤ 100 mg, Cells ≤ 20 mg (Approximately equal to 2×108bacterial, 2×107yeast, or 2×106mammalian cells) | ||||||
| Purity | High quality, inhibitor-free DNA. Typical A260/A280 ≥1.8 | ||||||
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. | ||||||
| Sample Storage | Eluted DNA should be stored at ≤ -20°C. | ||||||
| Size Range | Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield | ||||||
| Type | Total DNA | ||||||
| Yield | Up to 5 µg total DNA can be eluted into ≥ 10 µl | ||||||
A-105939, 22 Part Osteopathic Didactic Human Skull Axis Scientific
0090 Natural Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-105179, 22-Part Disarticulated Life-Size Human Skull Axis Scientific
A-105171 Half Disarticulated Human Skeleton Axis Scientific
A-104406 Complete Disarticulated Human Skeleton Axis Scientific
A-105759, Life-Size Human Skull With Removable Muscles Axis Scientific
0095F Fluorescent Pink Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
D4074- Quick-DNA Microprep Plus Kit- Zymo Research
A-105178, 3-Part Life-Size Human Skull With 8-Part Brain Axis Scientific
R1056- Quick-RNA Midiprep Kit 25 preps- Zymo Research 



