Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Maxiprep kit provides the fastest and simplest method available to efficiently isolate up to 1.2 mg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a novel binding system, the ZymoPURE II Plasmid Maxiprep kit can process up to 150 ml of culture in less than 20 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is bound onto a column with either a vacuum or a centrifuge instead of a lengthy gravity flow column. Additionally, there is no ethanol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 3 µg/µl), EndoZero, vaccine-grade, and transfection-ready. As an added convenience, the ZymoPURE II Plasmid Maxiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
| Technical specifications | |||||||
| Applicable For | Transfection, transformation, sequencing, restriction endonuclease digestion, in vitro transcription, in vivo studies, genome editing, and other sensitive applications. | ||||||
| Binding Capacity | 1.2 mg | ||||||
| Culture Input | ≤ 150 ml | ||||||
| Elution Volume | ≥ 200 µl | ||||||
| Endotoxin Levels | ≤ 0.025 EU/µg Plasmid DNA | ||||||
| Equipment | Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge. | ||||||
| Format | Spin Column | ||||||
| Processing Time | 20 min | ||||||
| Purity | Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0 | ||||||
| Recommended Sample Volume | ≤ 150 ml | ||||||
| Size Range | Up to 200 kb | ||||||
| Yield | ≤ 1.2 mg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized) | ||||||
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
0093 Green Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
4306 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Orange Bio Plas
A-105168 Miniature Human Skeleton Axis Scientific
A-105939, 22 Part Osteopathic Didactic Human Skull Axis Scientific
A-105179, 22-Part Disarticulated Life-Size Human Skull Axis Scientific
4300 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Natural Bio Plas
A-105178, 3-Part Life-Size Human Skull With 8-Part Brain Axis Scientific
SF 3300- STAT FAX 3300- Awareness Technology
A-104406 Complete Disarticulated Human Skeleton Axis Scientific
4303 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Blue Bio Plas
A-104267 Classic Human Skeleton With Study & Numbering Guide Axis Scientific
A-104281 Painted Human Skeleton Axis Scientific
A-105177 Painted And Numbered 3-Part Life-Size Human Skull Axis Scientific
4305 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Amber Bio Plas
A-105866 Miniature Skeleton With Flexible Spine Axis Scientific
Q720 WashLab770 Laboratory Bottle Washer Kimadi
0052 Snap On Tube Rack Cover, 96-Well, Polypropylene, Clear Natural Bio Plas
A-104264, 16-Part Mini Torso Axis Scientific
9136040 10x Phase Slider for use with P/N 3124041 (LP series) Labomed
0099 Black Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
D4074- Quick-DNA Microprep Plus Kit- Zymo Research 



