Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Maxiprep kit provides the fastest and simplest method available to efficiently isolate up to 1.2 mg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a novel binding system, the ZymoPURE II Plasmid Maxiprep kit can process up to 150 ml of culture in less than 20 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is bound onto a column with either a vacuum or a centrifuge instead of a lengthy gravity flow column. Additionally, there is no ethanol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 3 µg/µl), EndoZero, vaccine-grade, and transfection-ready. As an added convenience, the ZymoPURE II Plasmid Maxiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
| Technical specifications | |||||||
| Applicable For | Transfection, transformation, sequencing, restriction endonuclease digestion, in vitro transcription, in vivo studies, genome editing, and other sensitive applications. | ||||||
| Binding Capacity | 1.2 mg | ||||||
| Culture Input | ≤ 150 ml | ||||||
| Elution Volume | ≥ 200 µl | ||||||
| Endotoxin Levels | ≤ 0.025 EU/µg Plasmid DNA | ||||||
| Equipment | Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge. | ||||||
| Format | Spin Column | ||||||
| Processing Time | 20 min | ||||||
| Purity | Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0 | ||||||
| Recommended Sample Volume | ≤ 150 ml | ||||||
| Size Range | Up to 200 kb | ||||||
| Yield | ≤ 1.2 mg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized) | ||||||
A-105868, Human Skull With Masticatory Muscles Axis Scientific
WashLab790 Laboratory Bottle Washer Kimadi
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
A-105177 Painted And Numbered 3-Part Life-Size Human Skull Axis Scientific
A-105171 Half Disarticulated Human Skeleton Axis Scientific
A-105867, Human Skull Model With Flexible Neck Axis Scientific
A-105176 Disarticulated Half Child Skeleton Axis Scientific
A-105939, 22 Part Osteopathic Didactic Human Skull Axis Scientific
D4074- Quick-DNA Microprep Plus Kit- Zymo Research
840003 Digital Anemometer Thermometer Sper Scientific
9140300 Binocular tilting observation head 0° - 25° (excluding eyepieces) Labomed
BS-7000A Upright Fluorescent Biological Microscope BetScope
A-105179, 22-Part Disarticulated Life-Size Human Skull Axis Scientific
DR200 Natural Convection Oven Yamato
9132040 40x objective, spring, NA 0.65/WD 0.69mm (LP series DIN Phase Achromatic) Labomed
D4075- Quick-DNA Midiprep Plus Kit- Zymo Research
9126021 C-mount Adapter ½” Labomed
A-106193, Didactic Skull On Cervical Vertebrae With Nerves And Arteries Axis Scientific
V1002-S8 Optional eight channel adapter, fine tip stainless steel Accuris
9120010 10x/22mm WF eyepiece, focusable Labomed
QIAamp DNA Blood Mini Kit (250) - 51106 - Qiagen
9130010 10x/18mm WF eyepiece, focusable Labomed
9123010 10x objective, NA 0.25/WD 4.50mm (RP series Phase Plan Achromatic) Labomed
BS-7000B Inverted Fluorescent Biological Microscope BetScope
9126600 Trinocular Observation Head, 30° inclined (excluding eyepieces) Labomed
9123040 40x objective, spring, NA 0.65/WD 0.50mm (RP series Phase Plan Achromatic) Labomed
R1056- Quick-RNA Midiprep Kit 25 preps- Zymo Research
A-104264, 16-Part Mini Torso Axis Scientific
9120020 20x/12mm WF eyepiece, focusable Labomed
9136040 10x Phase Slider for use with P/N 3124041 (LP series) Labomed
A-104272 Life-Size Human Skull With Blood Vessels Axis Scientific
9010-0217 FED 400 UL Heating Chamber With Forced Convection Binder
9123100 100x objective, spring/oil, NA 1.25/WD 0.15mm (RP series Phase Plan Achromatic) Labomed
A-104281 Painted Human Skeleton Axis Scientific 


