The Mix & Go! E. coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation. Mix & Go! E. coli cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − Mix & Go! The premade Mix & Go! competent cells are highly efficient (> 108 transformants/µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.
| Technical specifications | |||||||
| Additional Info | Can be used for blue/white screening and is ideal for cDNA generation and library construction. | ||||||
| Genotype | F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara leu) 7697 galU galK rpsL nupG λ- | ||||||
| Processing Time | 20 Seconds | ||||||
| Product Storage | -70°C to -80°C | ||||||
| Transformation Efficiency | 108 – 109 transformants per µg of plasmid DNA | ||||||
D4074- Quick-DNA Microprep Plus Kit- Zymo Research
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
R1056- Quick-RNA Midiprep Kit 25 preps- Zymo Research
3970402- 4' Purifier Filtered PCR Enclosure with UV Light and Protection Panel- Labconco
A-104406 Complete Disarticulated Human Skeleton Axis Scientific
D4075- Quick-DNA Midiprep Plus Kit- Zymo Research
QIAamp DNA Blood Mini Kit (250) - 51106 - Qiagen
A-105170 Miniature Painted Human Skeleton Axis Scientific
3970323- 3' Purifier Vertical Clean Bench with Airflow Monitor- Labconco
0096F WELL MICROCENTRIFUGE TUBE RACK, FLUORESCENT YELLOW, 5/PK Bio Plas
28506-QIAquick PCR & Gel Cleanup Kit (100)- Qiagen
3970322- 3' Purifier Filtered PCR Enclosure with UV Light and Protection Panel- Labconco 


