The Mix & Go! E. coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation. Mix & Go! E. coli cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − Mix & Go! The premade Mix & Go! competent cells are highly efficient (> 108 transformants/µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.
| Technical specifications | |||||||
| Additional Info | Can be used for blue/white screening and is ideal for cDNA generation and library construction. | ||||||
| Genotype | F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara leu) 7697 galU galK rpsL nupG λ- | ||||||
| Processing Time | 20 Seconds | ||||||
| Product Storage | -70°C to -80°C | ||||||
| Transformation Efficiency | 108 – 109 transformants per µg of plasmid DNA | ||||||
800ES 1 Liter Blenders Waring
700612230- FreeZone 6 Liter -50C Console Freeze Dryer- Labconco
701212030- FreeZone 12 Liter -50C Console Freeze Dryer- Labconco
74116-RNeasy Mini QIAcube Kit (240)- Qiagen
700621030- FreeZone 6 Liter -50C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
3970401- 4' Purifier Vertical Clean Bench with UV Light and Protection Panel- Labconco
3970325- 3' Purifier Filtered PCR Enclosure with UV Light, Protection Panel and Airflow Monitor- Labconco
3970320- 3' Purifier Vertical Clean Bench- Labconco
700801030- FreeZone 8 Liter -50C Benchtop Freeze Dryer- Labconco
780601110-Bulk Tray Dryer- Labconco
3970400- 4' Purifier Vertical Clean Bench- Labconco
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
3970304- 3' Purifier Vertical Clean Bench with UV Light, Protection Panel and Airflow Monitor- Labconco
780601210-Bulk Tray Dryer- Labconco
701812210-FreeZone 18 Liter -50C Console Freeze Dryer- Labconco
700611230- FreeZone 6 Liter -50C Console Freeze Dryer- Labconco
3970323- 3' Purifier Vertical Clean Bench with Airflow Monitor- Labconco
3970321- 3' Purifier Vertical Clean Bench with UV Light and Protection Panel - Labconco
700621130- FreeZone 6 Liter -50C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
3970305- 3' Purifier Filtered PCR Enclosure with UV Light, Protection Panel and Airflow Monitor- Labconco
QIAamp DNA Blood Mini Kit (250) - 51106 - Qiagen
3970324- 3' Purifier Vertical Clean Bench with UV Light, Protection Panel and Airflow Monitor- Labconco
D4075- Quick-DNA Midiprep Plus Kit- Zymo Research 


