The Mix & Go! E. coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation. Mix & Go!E. coli cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − Mix & Go! The premade Mix & Go! competent cells are highly efficient (> 108 transformants/µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.
| Technical specifications | |||||||
| Additional Info | Can be used for general cloning and blue/white screening | ||||||
| Genotype | F’[traD36 lacIq Δ(lacZ) M15 proA+B+] glnV (supE) thi-1 Δ(mcrB-hsdSM)5 (rK- mK- McrB-) thi Δ(lac-proAB) | ||||||
| Processing Time | 20 Seconds | ||||||
| Product Storage | -70°C to -80°C | ||||||
| Transformation Efficiency | 108– 109transformants per µg of plasmid DNA | ||||||
710402000-FreeZone 4.5 Liter -84C Benchtop Freeze Dryer- Labconco
3970402- 4' Purifier Filtered PCR Enclosure with UV Light and Protection Panel- Labconco
3970323- 3' Purifier Vertical Clean Bench with Airflow Monitor- Labconco
20X Tris Buffered Saline with Tween 20, pH 7.4 - Hoefer
710201010-FreeZone 2.5 Liter -84C Benchtop Freeze Dryer- Labconco
794801110-Stoppering Tray Dryer- Labconco
3970303- 3' Purifier Vertical Clean Bench with Airflow Monitor- Labconco
710402010-FreeZone 4.5 Liter -84C Benchtop Freeze Dryer- Labconco
3970301- 3' Purifier Vertical Clean Bench with UV Light and Protection Panel- Labconco
710202010-FreeZone 2.5 Liter -84C Benchtop Freeze Dryer- Labconco
700622030- FreeZone 6 Liter -50C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
A-105868, Human Skull With Masticatory Muscles Axis Scientific
711221010-FreeZone 12 Liter -84C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
D4074- Quick-DNA Microprep Plus Kit- Zymo Research
711221110-FreeZone 12 Liter -84C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
701822130-FreeZone 18 Liter -50C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
701811230-FreeZone 18 Liter -50C Console Freeze Dryer- Labconco
711212110-FreeZone 12 Liter -84C Console Freeze Dryer- Labconco
0052 Snap On Tube Rack Cover, 96-Well, Polypropylene, Clear Natural Bio Plas
794801010-Stoppering Tray Dryer- Labconco
D4075- Quick-DNA Midiprep Plus Kit- Zymo Research 



