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T3003/T3005- Mix & Go! Competent Cells -JM109- Zymo Research

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The Mix & Go! E. coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation. Mix & Go! E. coli cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − Mix & Go! The premade Mix & Go! competent cells are highly efficient (> 108 transformants/ µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.

Technical specifications
Additional Info Partly restriction-deficient; good strain for cloning repetitive DNA (recA-). Suppresses many amber mutations when glutamine is available but not the S100 or S7 mutation of λ, e.g.,λgt11. Can be used for M13 cloning/sequencing and blue/white screening.
Genotype F`[traD36 proA+B+ laclq Δ(lacZ)M15] Δ(lac-proAB) glnV44 (supE44) e14- (McrA-) thi gyrA96 (NalR) endA1 hsdR17(rk- mk+) relA1 recA1
Processing Time 20 Seconds
Product Storage -70°C to -80°C
Transformation Efficiency 108 – 109 transformants per µg of plasmid DNA
SKU: T3003/T3005 Categories: , Tags: , Brand:
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T3003 (Mix and Go! Competent Cells -JM109 – 10 x 100 µl), T3005 (Mix and Go! Competent Cells -JM109 – 96 x 50 µl)

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