This assay has high sensitivity and excellent specificity for detecting Mouse Alanine Aminotransferase (ALT). The assay range is from 1.56 to 100 ng/ml (Sandwich kit) with a sensitivity of 0.61 ng/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.
- High sensitivity and specificity
- Perfect reproducibility and consistency across batches
- Quality control with three-level inspections
- Wide range of targets/species available
- Intra-assay: CV<10%; Inter-assay: CV<12%
Alanine transaminase or ALT is a transaminase enzyme. It is also called serum glutamic pyruvic transaminase (SGPT) or alanine aminotransferase (ALAT). ALT is found in serum and in various bodily tissues, but is most commonly associated with the liver. It catalyzes the transfer of an amino group from alanine to a-ketoglutarate, the products of this reversible transamination reaction being pyruvate and glutamate. It is commonly measured clinically as a part of a diagnostic liver function test, to determine liver health. GPT1 transcript is moderately expressed in kidney, liver, heart, and fat. Another GPT1 transcript shows a slightly different expression pattern, with a 3.9-kb transcript expressed at high levels in muscle, fat, kidney, and brain and at lower levels in liver and breast.
| Specifications | |||
| Assay duration | Multiple steps | ||
| Assay Type | Sandwich | ||
| Conjugate ELISA | Biotin | ||
| Format | Pre-coated | ||
| Host | Rabbit | ||
| Primary antibody reactivity | Mouse | ||
| Target protein | ALT | ||
| Size | 1 kit | ||
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids | ||
| Cross Reactivity | No significant cross-reactivity or interference between Lipoprotein lipase (LPL) and analogues was observed | ||
| Detection Method | Colorimetric | ||
| Time to Results | 3 h | ||
| Shelf Life | 12 Months | ||
| Detection Range | 1.56 – 100 ng/ml | ||
| Storage Temperature | 4 °C for one month (frequent use), −20 °C for one year | ||
| Sample Volume | 100 µl | ||
| Sensitivity | 0.61 ng/ml | ||
| Regulatory Status | RUO | ||
| No. of tests | 96 wells | ||
794801010-Stoppering Tray Dryer- Labconco
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
701812010-FreeZone 18 Liter -50C Console Freeze Dryer- Labconco
0096 Well Microcentrifuge Tube Rack, Yellow, 5/Pk
A-105176 Disarticulated Half Child Skeleton Axis Scientific
R1056- Quick-RNA Midiprep Kit 25 preps- Zymo Research
0092F Fluorescent Blue Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-105939, 22 Part Osteopathic Didactic Human Skull Axis Scientific
0097 Lavender Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-105168 Miniature Human Skeleton Axis Scientific
0094F Fluorescent Orange Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
20X Tris Buffered Saline with Tween 20, pH 7.4 - Hoefer
QIAamp DNA Blood Mini Kit (250) - 51106 - Qiagen
EZ-WR1 E-tank Wrench - E-Z Systems
3970223- 2' Purifier Vertical Clean Bench with Airflow Monitor- Labconco
CTL-REFM-PRSG -Refractometer- LW Scientific
0093F Fluorescent Green Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
28506-QIAquick PCR & Gel Cleanup Kit (100)- Qiagen
0090 Natural Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
D4074- Quick-DNA Microprep Plus Kit- Zymo Research
EZ-320 Oxygen Regulator - E-Z Systems 



