The Direct-zol™-96 MagBead RNA kit provides a high-throughput (96-well), magnetic bead-based isolation of high-quality RNA directly from samples in TRI Reagent® and similar. The extraction method inactivates viruses and other infectious agents. Total RNA including small/microRNAs (≥17 nt) are effectively isolated from a variety of sample sources (cells, tissues, biological liquids, etc.). The procedure is easy: simply add ethanol and MagBinding Beads to a sample in TRI Reagent®, wash and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including Next-Gen sequencing, RT-PCR, transcription profiling, hybridization, etc.
| Technical specifications | |||||||
| Compatibility | TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®. | ||||||
| Equipment | Microcentrifuge, vortex, magstand | ||||||
| Sample Inactivation | TRI Reagent® (provided with R2101, R2103, and R2105) inhibits RNase activity and inactivates viruses and other infectious agents. | ||||||
| Sample Source | Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)). | ||||||
| Size Range | Total RNA ≥ 17 nt | ||||||
| Yield | 10 µg RNA (binding capacity), ≥ 30 µl (elution volume) | ||||||
A-105178, 3-Part Life-Size Human Skull With 8-Part Brain Axis Scientific
0091 Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
0093 Green Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
RC 900 - Rotary Evaporator- Logos BioSystem
D4075- Quick-DNA Midiprep Plus Kit- Zymo Research
RE801C-W- Rotary Evaporator - Yamato
D4074- Quick-DNA Microprep Plus Kit- Zymo Research 


