The Direct-zol™-96 MagBead RNA kit provides a high-throughput (96-well), magnetic bead-based isolation of high-quality RNA directly from samples in TRI Reagent® and similar. The extraction method inactivates viruses and other infectious agents. Total RNA including small/microRNAs (≥17 nt) are effectively isolated from a variety of sample sources (cells, tissues, biological liquids, etc.). The procedure is easy: simply add ethanol and MagBinding Beads to a sample in TRI Reagent®, wash and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including Next-Gen sequencing, RT-PCR, transcription profiling, hybridization, etc.
| Technical specifications | |||||||
| Compatibility | TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®. | ||||||
| Equipment | Microcentrifuge, vortex, magstand | ||||||
| Sample Inactivation | TRI Reagent® (provided with R2101, R2103, and R2105) inhibits RNase activity and inactivates viruses and other infectious agents. | ||||||
| Sample Source | Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)). | ||||||
| Size Range | Total RNA ≥ 17 nt | ||||||
| Yield | 10 µg RNA (binding capacity), ≥ 30 µl (elution volume) | ||||||
S62 Premier Academic Series Skeleton, Male, Unpainted, Sacral Mount- Denoyer Geppert
HW-TMS-Teenie Mouse - Neonatal Mouse Pup Simulators- Hundred
D4074- Quick-DNA Microprep Plus Kit- Zymo Research
0715-00 Multi-Torso - Male & Female Inserts- Denoyer Geppert
F144067-PIPETMAN G P10mLG, 1-10 mL-Gilson
M-FEED-P -CORE SYSTEM consists of 4 items: bottom, lid, water, and feeder. Each item purchased separately. Mouse Feeders, Plastic Mouse Feeder (PET, no grate) - Innovive
700401030- FreeZone 4.5 Liter -50C Benchtop Freeze Dryer- Labconco
C806H-PowerSpin™ FX Centrifuge- Unico
F144056M-PIPETMAN G P20G, 2-20 µL, Metal Ejector-Gilson
3970401- 4' Purifier Vertical Clean Bench with UV Light and Protection Panel- Labconco
710402010-FreeZone 4.5 Liter -84C Benchtop Freeze Dryer- Labconco
HW-RSSK-Humaneway Rat Tail Vein Simulator- Hundred
D4075- Quick-DNA Midiprep Plus Kit- Zymo Research 



