The Direct-zol™-96 MagBead RNA kit provides a high-throughput (96-well), magnetic bead-based isolation of high-quality RNA directly from samples in TRI Reagent® and similar. The extraction method inactivates viruses and other infectious agents. Total RNA including small/microRNAs (≥17 nt) are effectively isolated from a variety of sample sources (cells, tissues, biological liquids, etc.). The procedure is easy: simply add ethanol and MagBinding Beads to a sample in TRI Reagent®, wash and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including Next-Gen sequencing, RT-PCR, transcription profiling, hybridization, etc.
| Technical specifications | |||||||
| Compatibility | TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®. | ||||||
| Equipment | Microcentrifuge, vortex, magstand | ||||||
| Sample Inactivation | TRI Reagent® (provided with R2101, R2103, and R2105) inhibits RNase activity and inactivates viruses and other infectious agents. | ||||||
| Sample Source | Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)). | ||||||
| Size Range | Total RNA ≥ 17 nt | ||||||
| Yield | 10 µg RNA (binding capacity), ≥ 30 µl (elution volume) | ||||||
QIAamp DNA Blood Mini Kit (250) - 51106 - Qiagen
SF2600- Stat Fax®2600- Awareness Technology
D4074- Quick-DNA Microprep Plus Kit- Zymo Research
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
4300 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Natural Bio Plas
D4075- Quick-DNA Midiprep Plus Kit- Zymo Research
A-105173 Miniature Skull Axis Scientific
4303 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Blue Bio Plas
0093 Green Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
R1056- Quick-RNA Midiprep Kit 25 preps- Zymo Research
A-104271, 3-Part Life-Size Painted Didactic Human Skull Axis Scientific
A-105168 Miniature Human Skeleton Axis Scientific
3970303- 3' Purifier Vertical Clean Bench with Airflow Monitor- Labconco
A-106193, Didactic Skull On Cervical Vertebrae With Nerves And Arteries Axis Scientific
A-104281 Painted Human Skeleton Axis Scientific
A-105867, Human Skull Model With Flexible Neck Axis Scientific
V1002-8 Optional eight channel adapter, for use with 200ul pipette tips Accuris
A-105177 Painted And Numbered 3-Part Life-Size Human Skull Axis Scientific
3970400- 4' Purifier Vertical Clean Bench- Labconco
0096F WELL MICROCENTRIFUGE TUBE RACK, FLUORESCENT YELLOW, 5/PK Bio Plas
A-105179, 22-Part Disarticulated Life-Size Human Skull Axis Scientific
A-105866 Miniature Skeleton With Flexible Spine Axis Scientific 


