The Direct-zol™-96 MagBead RNA kit provides a high-throughput (96-well), magnetic bead-based isolation of high-quality RNA directly from samples in TRI Reagent® and similar. The extraction method inactivates viruses and other infectious agents. Total RNA including small/microRNAs (≥17 nt) are effectively isolated from a variety of sample sources (cells, tissues, biological liquids, etc.). The procedure is easy: simply add ethanol and MagBinding Beads to a sample in TRI Reagent®, wash and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including Next-Gen sequencing, RT-PCR, transcription profiling, hybridization, etc.
| Technical specifications | |||||||
| Compatibility | TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®. | ||||||
| Equipment | Microcentrifuge, vortex, magstand | ||||||
| Sample Inactivation | TRI Reagent® (provided with R2101, R2103, and R2105) inhibits RNase activity and inactivates viruses and other infectious agents. | ||||||
| Sample Source | Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)). | ||||||
| Size Range | Total RNA ≥ 17 nt | ||||||
| Yield | 10 µg RNA (binding capacity), ≥ 30 µl (elution volume) | ||||||
0052 Snap On Tube Rack Cover, 96-Well, Polypropylene, Clear Natural Bio Plas
A-105176 Disarticulated Half Child Skeleton Axis Scientific
A-105868, Human Skull With Masticatory Muscles Axis Scientific
A-104270, 3-Part Life-Size Numbered Human Skull Axis Scientific
0093F Fluorescent Green Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
0095F Fluorescent Pink Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
QIAamp DNA Blood Mini Kit (250) - 51106 - Qiagen
0097 Lavender Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-104264, 16-Part Mini Torso Axis Scientific
A-105759, Life-Size Human Skull With Removable Muscles Axis Scientific
0096 Well Microcentrifuge Tube Rack, Yellow, 5/Pk
A-105852 Classic Flexible Human Skeleton Axis Scientific
0092 Blue Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
A-105939, 22 Part Osteopathic Didactic Human Skull Axis Scientific
A-105173 Miniature Skull Axis Scientific
0099 Black Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
Q720 WashLab770 Laboratory Bottle Washer Kimadi
A-104272 Life-Size Human Skull With Blood Vessels Axis Scientific
4306 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Orange Bio Plas
A-104406 Complete Disarticulated Human Skeleton Axis Scientific
A-105172 Human Skeleton With Flexible Spine, Muscle Insertions, And Ligaments Axis Scientific 



