The Direct-zol-96 RNA Kits are RNA purification kits that provide a streamlined method for the purification of up to 10 µg (per well) of high-quality RNA directly from samples in TRI Reagent®. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.) using this product. The extraction method inactivates viruses and other infectious agents. The procedure is easy: simply apply a sample in TRI Reagent® to the Zymo-Spin I-96 Plate, then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc. The entire procedure typically takes about 30 minutes (per 2 plates).
| Technical specifications | |||||||
| Compatibility | TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®. | ||||||
| Equipment | Microcentrifuge, vortex | ||||||
| Sample Inactivation | TRI Reagent® (provided with R2055, R2057) inhibits RNase activity and inactivates viruses and other infectious agents. | ||||||
| Sample Source | Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)). | ||||||
| Size Range | Total RNA ≥ 17 nt | ||||||
| Yield | 10 µg RNA (binding capacity), ≥10 µl (elution volume) | ||||||
A-104405, 3-Part Life-Size Human Skull Axis Scientific
D4074- Quick-DNA Microprep Plus Kit- Zymo Research
0093 Green Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
0091 Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
3970205- 2' Purifier Filtered PCR Enclosure with UV Light, Protection Panel and Airflow Monitor- Labconco
0090 Natural Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
RE801C-W- Rotary Evaporator - Yamato
0052 Snap On Tube Rack Cover, 96-Well, Polypropylene, Clear Natural Bio Plas
QIAamp DNA Blood Mini Kit (250) - 51106 - Qiagen
R1056- Quick-RNA Midiprep Kit 25 preps- Zymo Research
VP200 Vacuum Pump, Universal VAC Hoefer
A-104271, 3-Part Life-Size Painted Didactic Human Skull Axis Scientific
GR152-10 - Ammonium Persulfate, ACS Reagent Grade- Hoefer
A-104281 Painted Human Skeleton Axis Scientific
0094F Fluorescent Orange Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
RE301B-W - Rotary evaporator, Glass "B", Water Bath- Yamato
V0020-F1 Hydrophobic filter, pk/5 ( 1 each included) Accuris
BSH100-05 Block, 24 x 0.5ml Benchmark Scientific
GR149-1 -Tris-Glycin e-SDS Buffer, 10X Solution (Ultrapure) 1LT- Hoefer
0095 Pink Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
PS300B 300 Volt Power Supply Hoefer
A-105176 Disarticulated Half Child Skeleton Axis Scientific 



