The Direct-zol-96 RNA Kits are RNA purification kits that provide a streamlined method for the purification of up to 10 µg (per well) of high-quality RNA directly from samples in TRI Reagent®. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.) using this product. The extraction method inactivates viruses and other infectious agents. The procedure is easy: simply apply a sample in TRI Reagent® to the Zymo-Spin I-96 Plate, then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc. The entire procedure typically takes about 30 minutes (per 2 plates).
| Technical specifications | |||||||
| Compatibility | TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®. | ||||||
| Equipment | Microcentrifuge, vortex | ||||||
| Sample Inactivation | TRI Reagent® (provided with R2055, R2057) inhibits RNase activity and inactivates viruses and other infectious agents. | ||||||
| Sample Source | Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)). | ||||||
| Size Range | Total RNA ≥ 17 nt | ||||||
| Yield | 10 µg RNA (binding capacity), ≥10 µl (elution volume) | ||||||
0052 Snap On Tube Rack Cover, 96-Well, Polypropylene, Clear Natural Bio Plas
A-105852 Classic Flexible Human Skeleton Axis Scientific
A-105940, 22 Part Osteopathic Natural Bone Human Skull Axis Scientific
R1056- Quick-RNA Midiprep Kit 25 preps- Zymo Research
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
0091 Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
R1009-Quick-DNA/RNA FFPE Miniprep Kit 50 Preps- Zymo Research
A-105868, Human Skull With Masticatory Muscles Axis Scientific
V1002-S8 Optional eight channel adapter, fine tip stainless steel Accuris
D4075- Quick-DNA Midiprep Plus Kit- Zymo Research
RE301B-WV - Rotary evaporator, Glass "B", Water Bath, VR300 - Yamato
A-104406 Complete Disarticulated Human Skeleton Axis Scientific
701211030- FreeZone 12 Liter -50C Console Freeze Dryer - Labconco
GR144-100 -TE (Tris-EDTA) Buffer, 50X Solution, Ultrapure 100 ML- Hoefer
D4074- Quick-DNA Microprep Plus Kit- Zymo Research 


