The Direct-zol-96 RNA Kits are RNA purification kits that provide a streamlined method for the purification of up to 10 µg (per well) of high-quality RNA directly from samples in TRI Reagent®. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.) using this product. The extraction method inactivates viruses and other infectious agents. The procedure is easy: simply apply a sample in TRI Reagent® to the Zymo-Spin I-96 Plate, then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc. The entire procedure typically takes about 30 minutes (per 2 plates).
| Technical specifications | |||||||
| Compatibility | TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®. | ||||||
| Equipment | Microcentrifuge, vortex | ||||||
| Sample Inactivation | TRI Reagent® (provided with R2055, R2057) inhibits RNase activity and inactivates viruses and other infectious agents. | ||||||
| Sample Source | Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)). | ||||||
| Size Range | Total RNA ≥ 17 nt | ||||||
| Yield | 10 µg RNA (binding capacity), ≥10 µl (elution volume) | ||||||
3970403- 4' Purifier Vertical Clean Bench with Airflow Monitor- Labconco
C8304-PowerSpin™ HX Centrifuge- Unico
3120010-802 Micrometer reticule, scale 10mm divided in 100 parts, X-axis horizontal scale with locking circlip Labomed
701811110-FreeZone 18 Liter -50C Console Freeze Dryer- Labconco
3970202- 2' Purifier Filtered PCR Enclosure with UV Light and Protection Panel- Labconco
0714-00 Multi-Torso - Female Only Denoyer-Geppert
4303 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Blue Bio Plas
19781-QIAfilter Mega-Giga Cartridges (5)- Qiagen
4307 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Lavender Bio Plas
1950, T8DCS Double Beam UV-Vis Persee
3970420- 4' Purifier Vertical Clean Bench- Labconco
4302 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Yellow Bio Plas
4304 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Green Bio Plas
4310 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Lavender Bio Plas
06-209-50 - 50 Ml Omni Prep Tube Holder- Omni International
06-021 - Omni Prep- Omni International
06-209-05 - 5 Ml Omni Prep Tube Holder- Omni International
0099 Black Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
06-209-02 - 1.5 To 2 Ml Omni Prep Tube Holder- Omni International
SE915 Large Format PAGE Multiple Gel Caster for Use with SE9102 Cassettes Hoefer
A-105178, 3-Part Life-Size Human Skull With 8-Part Brain Axis Scientific
R1002-YeaStar RNA Kit 40 Preps- Zymo Research
R2024-Quick-RNA Plant Miniprep Kit 50 Preps- Zymo Research
06-210 - Cooling Ice Tray - For 15 Ml - 50 Ml Tubes- Omni International
06-207 - Cooling Ice Tray - For 2 Ml - 5 Ml Tubes- Omni International
06-209-15 - 15 Ml Omni Prep Tube Holder- Omni International
R2001- ZymoBIOMICS RNA Miniprep Kit 50 Preps- Zymo Research
D4075- Quick-DNA Midiprep Plus Kit- Zymo Research
A-104267 Classic Human Skeleton With Study & Numbering Guide Axis Scientific
D4074- Quick-DNA Microprep Plus Kit- Zymo Research
TE62 Standard Transfer Tank with Cooling Chamber Hoefer
A-104405, 3-Part Life-Size Human Skull Axis Scientific
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
PS300B 300 Volt Power Supply Hoefer
R1056- Quick-RNA Midiprep Kit 25 preps- Zymo Research
A-105759, Life-Size Human Skull With Removable Muscles Axis Scientific
A-105173 Miniature Skull Axis Scientific
A-104271, 3-Part Life-Size Painted Didactic Human Skull Axis Scientific
A-105852 Classic Flexible Human Skeleton Axis Scientific
A-105172 Human Skeleton With Flexible Spine, Muscle Insertions, And Ligaments Axis Scientific
Q620 WashLab760 Laboratory Bottle Washer Kimadi
A-106193, Didactic Skull On Cervical Vertebrae With Nerves And Arteries Axis Scientific
A-105170 Miniature Painted Human Skeleton Axis Scientific
A-105866 Miniature Skeleton With Flexible Spine Axis Scientific
T3041 - Mix & Go! XJb Autolysis Competent Cells, 1 ml 500x L-Arabinose - 10 x 100 µl- Zymo Research
0093 Green Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
T3051 - Mix & Go! XJb (DE3) Autolysis Competent Cells, 1 ml 500x L-Arabinose 10 x 100 µl- Zymo Research
T3017 - Mix & Go! Competent Cells-TG1, 10 x 100 µl- Zymo Research 



