The ZymoBIOMICS DNA/RNA Miniprep Kit is designed for purifying DNA and RNA from a wide array of sample inputs (e.g. feces, soil, plant, water, and biofilms) that is ready for microbiome or metagenome analyses. The ZymoBIOMICS innovative lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae). The provided DNA/RNA Shield preserves nucleic acids at ambient temperatures, providing an unbiased molecular snapshot of the sample. The procedure uses Zymo-Spin Column technology that results in high-quality DNA and total RNA (including small RNAs 17-200 nt) that is free of PCR inhibitors (e.g. polyphenols, humic acids, and fulvic acids). Ready for RT-PCR, arrays, sequencing, etc.
| Technical specifications | |||||||
| Equipment | Microcentrifuge, vortex, cell disrupter (recommended) | ||||||
| Purity | DNA and RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8. | ||||||
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host RNA is efficiently isolated from ≤ 200 mg of mammalian feces, ≤ 250 mg soil, ≤ 200 mg plant/seed, 50-100 mg (wet weight) fungal bacterial cells, biofilms, and water. | ||||||
| Size Range | DNA and Total RNA ≥17 nt | ||||||
| Yield | 100 µg DNA/RNA (binding capacity), ≥50 µl (elution volume) | ||||||
D4074- Quick-DNA Microprep Plus Kit- Zymo Research
3970304- 3' Purifier Vertical Clean Bench with UV Light, Protection Panel and Airflow Monitor- Labconco
701821010-FreeZone 18 Liter -50C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
700201030- FreeZone 2.5 Liter -50C Benchtop Freeze Dryer- Labconco
0095F Fluorescent Pink Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
QIAamp DNA Blood Mini Kit (250) - 51106 - Qiagen
700622030- FreeZone 6 Liter -50C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
701211110- FreeZone 12 Liter -50C Console Freeze Dryer- Labconco
R1056- Quick-RNA Midiprep Kit 25 preps- Zymo Research
3970325- 3' Purifier Filtered PCR Enclosure with UV Light, Protection Panel and Airflow Monitor- Labconco
701811110-FreeZone 18 Liter -50C Console Freeze Dryer- Labconco
710621030-FreeZone 6 Liter -84C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
3970400- 4' Purifier Vertical Clean Bench- Labconco
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
3970405- 4' Purifier Filtered PCR Enclosure with UV Light, Protection Panel and Airflow Monitor- Labconco
710621100-FreeZone 6 Liter -84C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
711211210-FreeZone 12 Liter -84C Console Freeze Dryer- Labconco
0093F Fluorescent Green Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
700612030- FreeZone 6 Liter -50C Console Freeze Dryer- Labconco
3970324- 3' Purifier Vertical Clean Bench with UV Light, Protection Panel and Airflow Monitor- Labconco
701811130-FreeZone 18 Liter -50C Console Freeze Dryer- Labconco
701212230- FreeZone 12 Liter -50C Console Freeze Dryer- Labconco
3970305- 3' Purifier Filtered PCR Enclosure with UV Light, Protection Panel and Airflow Monitor- Labconco
3970303- 3' Purifier Vertical Clean Bench with Airflow Monitor- Labconco
701221030- FreeZone 12 Liter -50C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
0091 Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
780601110-Bulk Tray Dryer- Labconco
3970420- 4' Purifier Vertical Clean Bench- Labconco
710622110-FreeZone 6 Liter -84C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
700612130- FreeZone 6 Liter -50C Console Freeze Dryer- Labconco
700621130- FreeZone 6 Liter -50C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
780601210-Bulk Tray Dryer- Labconco
3970404- 4' Purifier Vertical Clean Bench with UV Light, Protection Panel and Airflow Monitor- Labconco
701811230-FreeZone 18 Liter -50C Console Freeze Dryer- Labconco
711212010-FreeZone 12 Liter -84C Console Freeze Dryer- Labconco
3970321- 3' Purifier Vertical Clean Bench with UV Light and Protection Panel - Labconco
3970322- 3' Purifier Filtered PCR Enclosure with UV Light and Protection Panel- Labconco
700622130- FreeZone 6 Liter -50C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
701812070-FreeZone 18 Liter -50C Console Freeze Dryer- Labconco
3970224- 2' Purifier Vertical Clean Bench with UV Light, Protection Panel and Airflow Monitor- Labconco
3970205- 2' Purifier Filtered PCR Enclosure with UV Light, Protection Panel and Airflow Monitor- Labconco
711211010-FreeZone 12 Liter -84C Console Freeze Dryer- Labconco 

