The YeaStar RNA Kit provides all the necessary reagents for RNA isolation from a broad spectrum of fungi including: Aspergillus fumigatus, Aspergillus nidulans, Aspergillus nivens var. aureus, Candida albicans/, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe. Generally, the kit can be used for the purification of high-quality, total RNA from any fungus that can be lysed by yeast lytic enzyme. The kit facilitates the purification of 10-25 µg of total RNA from 1-1.5 ml of cultured cells using innovative Zymo-Spin column technology.
| Technical specifications | |||||||
| Applicable For | Isolated RNA is well suited for downstream applications such as Northern blotting, RT-PCR, Next-Generation Sequencing, Microarray, hybridization and other sensitive applications | ||||||
| Elution Volume | ≥ 60 µl DNase/RNase-Free Water | ||||||
| Equipment | Microcentrifuge & Heat Block/Bath | ||||||
| Processing Time | 30 minutes | ||||||
| Processing Volume | ≤ 1.5 ml of Culture (1-5 x 107cells) | ||||||
| Purity | Typical A260/A280 & A260/A230 ≥ 1.8 | ||||||
| Sample Source | Cell Culture (Colonies/Patches or Liquid Culture) | ||||||
| Size Range | ≥ 17 nt | ||||||
| Type | Total RNA | ||||||
| Yield | ≤ 25 µg of total RNA | ||||||
3970400- 4' Purifier Vertical Clean Bench- Labconco
D4075- Quick-DNA Midiprep Plus Kit- Zymo Research
RE301A-WV - Rotary evaporator, Glass "A", Water Bath, VR300- Yamato
0096 Well Microcentrifuge Tube Rack, Yellow, 5/Pk
A-105176 Disarticulated Half Child Skeleton Axis Scientific
A-104270, 3-Part Life-Size Numbered Human Skull Axis Scientific
0094 Orange Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-105867, Human Skull Model With Flexible Neck Axis Scientific
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
A-105940, 22 Part Osteopathic Natural Bone Human Skull Axis Scientific
RE301A-O - Rotary evaporator, Glass "A", Oil Bath- Yamato
A-105866 Miniature Skeleton With Flexible Spine Axis Scientific
0091F Assorted Fluorescent Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
WashLab790 Laboratory Bottle Washer Kimadi
R1056- Quick-RNA Midiprep Kit 25 preps- Zymo Research
A-105170 Miniature Painted Human Skeleton Axis Scientific
AZI600-Azure 600-Azure BioSystems
A-105171 Half Disarticulated Human Skeleton Axis Scientific
A-105168 Miniature Human Skeleton Axis Scientific
4000 Blue Polypropylene Snap Cap Microcentrifuge Tube Opening Tool (Case Of 100) Bio Plas
3970302- 3' Purifier Filtered PCR Enclosure with UV Light and Protection Panel- Labconco
A-105179, 22-Part Disarticulated Life-Size Human Skull Axis Scientific
A-104272 Life-Size Human Skull With Blood Vessels Axis Scientific
A-105939, 22 Part Osteopathic Didactic Human Skull Axis Scientific 


