The YeaStar RNA Kit provides all the necessary reagents for RNA isolation from a broad spectrum of fungi including: Aspergillus fumigatus, Aspergillus nidulans, Aspergillus nivens var. aureus, Candida albicans/, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe. Generally, the kit can be used for the purification of high-quality, total RNA from any fungus that can be lysed by yeast lytic enzyme. The kit facilitates the purification of 10-25 µg of total RNA from 1-1.5 ml of cultured cells using innovative Zymo-Spin column technology.
| Technical specifications | |||||||
| Applicable For | Isolated RNA is well suited for downstream applications such as Northern blotting, RT-PCR, Next-Generation Sequencing, Microarray, hybridization and other sensitive applications | ||||||
| Elution Volume | ≥ 60 µl DNase/RNase-Free Water | ||||||
| Equipment | Microcentrifuge & Heat Block/Bath | ||||||
| Processing Time | 30 minutes | ||||||
| Processing Volume | ≤ 1.5 ml of Culture (1-5 x 107cells) | ||||||
| Purity | Typical A260/A280 & A260/A230 ≥ 1.8 | ||||||
| Sample Source | Cell Culture (Colonies/Patches or Liquid Culture) | ||||||
| Size Range | ≥ 17 nt | ||||||
| Type | Total RNA | ||||||
| Yield | ≤ 25 µg of total RNA | ||||||
0094 Orange Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
0096F WELL MICROCENTRIFUGE TUBE RACK, FLUORESCENT YELLOW, 5/PK Bio Plas
0095F Fluorescent Pink Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
PT312-5M Plattforms Adam Equipment
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
QIAamp DNA Blood Mini Kit (250) - 51106 - Qiagen
D4074- Quick-DNA Microprep Plus Kit- Zymo Research
PT 312-10 Plattforms Adam Equipment
0090 Natural Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-104271, 3-Part Life-Size Painted Didactic Human Skull Axis Scientific
A-105173 Miniature Skull Axis Scientific
D4075- Quick-DNA Midiprep Plus Kit- Zymo Research
EZ-155-KF - Stand-Alone Vaporizer with Key Fill (KF Adapter Not Included) - E-Z Systems
0097 Lavender Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
EZ-150C- Classic Anesthesia Machine with Funnel Fill TEC 3 Vaporizer - E-Z Systems
EZ-230CL Oxygen Flow Regulator 0.5 – 8 LPM CGA-870 Connection - E-Z Systems
WashLab790 Laboratory Bottle Washer Kimadi
4303 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Blue Bio Plas
A-105176 Disarticulated Half Child Skeleton Axis Scientific
701811230-FreeZone 18 Liter -50C Console Freeze Dryer- Labconco
EZ-155- Stand-Alone Vaporizer with Funnel Fill - E-Z Systems
4300 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Natural Bio Plas
840003 Digital Anemometer Thermometer Sper Scientific
A-104272 Life-Size Human Skull With Blood Vessels Axis Scientific
701812210-FreeZone 18 Liter -50C Console Freeze Dryer- Labconco
EZ-190F - Auto Flow Vaporizer Machine - E-Z Systems
R1056- Quick-RNA Midiprep Kit 25 preps- Zymo Research 


