The YeaStar RNA Kit provides all the necessary reagents for RNA isolation from a broad spectrum of fungi including: Aspergillus fumigatus, Aspergillus nidulans, Aspergillus nivens var. aureus, Candida albicans/, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe. Generally, the kit can be used for the purification of high-quality, total RNA from any fungus that can be lysed by yeast lytic enzyme. The kit facilitates the purification of 10-25 µg of total RNA from 1-1.5 ml of cultured cells using innovative Zymo-Spin column technology.
| Technical specifications | |||||||
| Applicable For | Isolated RNA is well suited for downstream applications such as Northern blotting, RT-PCR, Next-Generation Sequencing, Microarray, hybridization and other sensitive applications | ||||||
| Elution Volume | ≥ 60 µl DNase/RNase-Free Water | ||||||
| Equipment | Microcentrifuge & Heat Block/Bath | ||||||
| Processing Time | 30 minutes | ||||||
| Processing Volume | ≤ 1.5 ml of Culture (1-5 x 107cells) | ||||||
| Purity | Typical A260/A280 & A260/A230 ≥ 1.8 | ||||||
| Sample Source | Cell Culture (Colonies/Patches or Liquid Culture) | ||||||
| Size Range | ≥ 17 nt | ||||||
| Type | Total RNA | ||||||
| Yield | ≤ 25 µg of total RNA | ||||||
A-105179, 22-Part Disarticulated Life-Size Human Skull Axis Scientific
0097 Lavender Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
0095F Fluorescent Pink Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-105866 Miniature Skeleton With Flexible Spine Axis Scientific
0092F Fluorescent Blue Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
D4075- Quick-DNA Midiprep Plus Kit- Zymo Research
A-105867, Human Skull Model With Flexible Neck Axis Scientific
V1002 Handheld vacuum controller with single channel adapters Accuris
RE601C-W - Rotary EvaporatorRE601C-W - Yamato
A-105171 Half Disarticulated Human Skeleton Axis Scientific
BSW13 Block For Digital Dry Bath - 20 x 12mm or 20 x 13mm Test Tubes Benchmark Scientific
A-105940, 22 Part Osteopathic Natural Bone Human Skull Axis Scientific
28506-QIAquick PCR & Gel Cleanup Kit (100)- Qiagen
0096 Well Microcentrifuge Tube Rack, Yellow, 5/Pk
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
D4074- Quick-DNA Microprep Plus Kit- Zymo Research 



