The YeaStar RNA Kit provides all the necessary reagents for RNA isolation from a broad spectrum of fungi including: Aspergillus fumigatus, Aspergillus nidulans, Aspergillus nivens var. aureus, Candida albicans/, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe. Generally, the kit can be used for the purification of high-quality, total RNA from any fungus that can be lysed by yeast lytic enzyme. The kit facilitates the purification of 10-25 µg of total RNA from 1-1.5 ml of cultured cells using innovative Zymo-Spin column technology.
| Technical specifications | |||||||
| Applicable For | Isolated RNA is well suited for downstream applications such as Northern blotting, RT-PCR, Next-Generation Sequencing, Microarray, hybridization and other sensitive applications | ||||||
| Elution Volume | ≥ 60 µl DNase/RNase-Free Water | ||||||
| Equipment | Microcentrifuge & Heat Block/Bath | ||||||
| Processing Time | 30 minutes | ||||||
| Processing Volume | ≤ 1.5 ml of Culture (1-5 x 107cells) | ||||||
| Purity | Typical A260/A280 & A260/A230 ≥ 1.8 | ||||||
| Sample Source | Cell Culture (Colonies/Patches or Liquid Culture) | ||||||
| Size Range | ≥ 17 nt | ||||||
| Type | Total RNA | ||||||
| Yield | ≤ 25 µg of total RNA | ||||||
QIAamp DNA Blood Mini Kit (250) - 51106 - Qiagen
0093 Green Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
4305 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Amber Bio Plas
4000 Blue Polypropylene Snap Cap Microcentrifuge Tube Opening Tool (Case Of 100) Bio Plas
D4074- Quick-DNA Microprep Plus Kit- Zymo Research
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
0092 Blue Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-105171 Half Disarticulated Human Skeleton Axis Scientific
0096F WELL MICROCENTRIFUGE TUBE RACK, FLUORESCENT YELLOW, 5/PK Bio Plas
A-105178, 3-Part Life-Size Human Skull With 8-Part Brain Axis Scientific
A-104270, 3-Part Life-Size Numbered Human Skull Axis Scientific
RE301C-WV - Rotary evaporator, Glass "C", Water Bath, VR300 - Yamato 



