R045B/R045A-PrimeSTAR Max DNA Polymerase—fast and high-fidelity PCR- Takara

Advantage 2 Polymerase Mix is an optimized blend of PCR enzymes that is a proven balance of high yield and high fidelity (with 3X higher fidelity than regular Taq) for cDNA amplification and library construction. With an automatic hot-start feature, our Advantage 2 Polymerase Mix can readily amplify a wide range of DNA templates, including long templates up to 18 kb, and complex genomic DNA up to 6 kb. The Advantage 2 Polymerase Mix includes two optimized buffers. dNTPs need to be purchased separately.

PrimeSTAR Max DNA Polymerase has the highest fidelity and fastest extension rate of any Takara Bio enzyme. The formulation is based on Takara’s unique, high-fidelity PrimeSTAR HS DNA Polymerase combined with an elongation factor to provide efficient priming and extension, which greatly reduces the time required for annealing and extension steps. As a result, PrimeSTAR Max DNA Polymerase can be used for exceptionally fast PCR. It is formulated as a 2X premix containing optimized buffer and dNTPs, which allows for quick preparation of reactions for high-throughput applications.

In addition, standardization of the extension step time makes PrimeSTAR Max DNA Polymerase suitable for reactions with excessive nucleic acids that would ordinarily be difficult to amplify due to non-specific binding. The superior processivity of PrimeSTAR Max DNA Polymerase prevents inhibition by excessive nucleic acids, resulting in a much higher success rate for PCR with minimal required optimization. The antibody-mediated hot-start feature provides improved specificity by preventing false initiation events during the reaction assembly.

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