- Product No.MAA133Hu22
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)C1
- Ig IsotypeIgG1 Kappa
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- ImmunogenRPA133Hu01-Recombinant Tumor Necrosis Factor Alpha (TNFa)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration1mg/mL
- Organism Species MoreMus musculus (Mouse)
- ApplicationsICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies.
SPECIFITY
The antibody is a mouse monoclonal antibody raised against TNFa. It has been selected for its ability to recognize TNFa in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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