View cart “QIAamp DNA Blood Mini Kit (250) – 51106 – Qiagen” has been added to your cart.

E2018-50/E2018-200- dsDNA Shearase Plus – Zymo Research

Name: E2018-50/E2018-200- dsDNA Shearase Plus - Zymo Research
Brand: Zymo Research
SKU: E2018-50/E2018-200
Availability: InStock

The product is already in the wishlist! Browse Wishlist

Compare

$128.00 – $467.00

The product is already in the wishlist! Browse Wishlist

Description
Specification

dsDNA Shearase Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. It has a particularly strong preference for dsDNA and generates random-ended DNA fragments of the desired size in a single step. This enzyme is compatible with low volume inputs, thus minimizing sample loss.

Technical specifications
Concentration		1 U/µl
Enzyme Inactivation		Heat inactivate enzyme at 65°C for 5 min.
Storage		Store at -20°C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70°C.
Unit Definition		One unit (1 U) is defined at the amount of enzyme required to convert 250 ng human DNA into DNA fragments in the range of 100-500 bp in 20 minutes at 42°C in total reaction volume in 10 µl.