DNA Degradase from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleotide components. DNA Degradase is ideal for whole-genome DNA methylation analysis by many downstream applications (i.e., HPLC, TLC, etc.). Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.
| Technical specifications | |||||||
| Assay Condition | DNA Degradase in 1X DNA Degradase Reaction Buffer. Incubate reaction mixtures at 37C for ≥ 1 hour. | ||||||
| Concentration | 10 U/µl | ||||||
| Enzyme Inactivation | 70C for 20 minutes | ||||||
| Storage | Store at -20C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70C. | ||||||
| Unit Definition | One unit (U) is the amount of enzyme required to degrade 1 µg of λ | ||||||
| Product | E2016 (DNA Degradase – 500 U), E2017 (DNA Degradase – 2000 U) |
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