ZymoBIOMICS Spike-in Control II (Low Microbial Load) consists of three bacteria strains, Truepera radiovictrix, Imtechella halotolerans and Allobacillus halotolerans. When spiked into a microbial sample, this product will serve as an in situ positive control for DNA-sequencing-based microbiome measurements. Of the three chosen bacteria, Imtechella halotolerans is Gram-negative and Allobacillus halotolerans is Gram-positive. Truepera radiovictrix is resistant to lysozyme lysis and has very high GC content. They represent different challenges in NGS-based analysis. Moreover, with accurately quantified cell number and a log abundance distribution, this standard enables absolute cell number quantification in cases such as pathogen load detection.
| Technical specifications | |||||||
| Biosafety | This product is not biohazardous as microbes have been fully inactivated. | ||||||
| Impurity Level | < 0.01% foreign microbial DNA | ||||||
| Sample Storage | -80°C | ||||||
| Storage Solution | DNA/RNA Shield™ | ||||||
H5000-05 Block, 54 x 0.5ml Benchmark Scientific
F123770-PIPETMAN Fixed F2, 2 µL-Gilson
H38878-0010, DRY ICE MAKER; U.S. MODEL, 12 X 6½ X 5 IN. Bel-Art
H1001-M - Incu-Shaker™ Mini With Non-Slip Rubber Mat - Benchmark Scientific
F144055M-PIPETMAN G P10G, 1-10 µL, Metal Ejector-Gilson
DKN-812C - Forced Convection Oven Programmable 300L 220V- Yamato
GR142-100 -N,N’ - Methylene-bis-Acrylamide 100gr- Hoefer
F144058M-PIPETMAN G P200G, 20-200 µL, Metal Ejector-Gilson
GR152-10 - Ammonium Persulfate, ACS Reagent Grade- Hoefer
MAA095HU21-Anti-MIP3a Mouse Monoclonal Antibody [clone: A4]-Cloud-Clone
F123607-PIPETMAN Fixed F5000, 5 mL-Gilson
H2010- Incu-Shaker™ 10l With Non-Slip Rubber Mat - Benchmark Scientific
L-CU60 -Compact Incubator- Unico
D5101 - Methylated-DNA IP Kit 10 Rxns.- Zymo Research
BCLO3034 IQ/OQ protocol For GAS SYSTEM (English version) Alliance Bio Expertise
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
D4074- Quick-DNA Microprep Plus Kit- Zymo Research
R1056- Quick-RNA Midiprep Kit 25 preps- Zymo Research
QIAamp DNA Blood Mini Kit (250) - 51106 - Qiagen
M-BTM -CORE SYSTEM consists of 4 items: bottom, lid, water, and feeder. Each item purchased separately. Mouse Feeders, Standard Mouse Feeder, extended-Use (PS, zinc plated steel insert grate) - Innovive
RVX5 - Rat Caging, Innocage® Lids External Water Bottle, Autowatering Innocage® IVC Rat Dual Filter - Innovive
MVX5 - CORE SYSTEM consists of 4 items: bottom, lid, water, and feeder. Each item purchased separately, Innocage® Ventilated Lids , Autowatering Innocage® IVC Mouse Dual Filter - Innovive
R-BTMA-H -Rat Caging, Innocage® Bottom, Autowatering cage bottom, empty - Innovive 



