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D5220-1- Micrococcal Nuclease – Zymo Research

Name: D5220-1- Micrococcal Nuclease - Zymo Research
Brand: Zymo Research
SKU: D5220-1
Price: 27.00 USD
Availability: InStock

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Description

Micrococcal Nuclease cleaves single-stranded and double-stranded DNA and RNA. Complete digestion with Micrococcal Nuclease yields mono- and oligonucleotides with 3′-phosphates.

Technical specifications
Concentration		0.1 U/µl
Enzyme Commission Number		(E.C. 3.1.31.1)
Enzyme Inactivation		EDTA or EGTA in molar excess of CaCl2.
Storage		Store at -20°C for up to 12 months. Avoid repeated freeze/thawing. Prolonged storage should be ≤ -70 °C.
Unit Definition		One unit (U) will produce 1.0 µmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37°C, based on EM/260 = 10,000 for the mixed nucleotides.