The Methylated-DNA IP Kit is designed for enrichment of 5mC-containing DNA from any pool of fragmented genomic DNA for use in genome-wide methylation analysis. It features a highly specific Anti-5-Methylcytosine Monoclonal Antibody for the immunoprecipitation of methylated DNA in only a few hours. This kit is capable of achieving over one hundred-fold enrichment of methylated DNA vs. non-methylated DNA. Recovered DNA is suitable for many downstream applications to analyze genome-wide DNA methylation including PCR, bisulfite treatment, whole-genome amplification, ultra-deep sequencing, and microarray. Control DNA and primers are included to monitor the success of the assay.
| Technical specifications | |||||||
| Applicable For | Immunoprecipitation of methylated DNA, PCR, sequencing, microarrays, etc. | ||||||
| Elution Volume | ≥ 15 µl | ||||||
| Input | 50 – 500 ng of DNA | ||||||
| Processing Time | 4 hours | ||||||
BSW13 Block For Digital Dry Bath - 20 x 12mm or 20 x 13mm Test Tubes Benchmark Scientific
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
D4075- Quick-DNA Midiprep Plus Kit- Zymo Research
D4074- Quick-DNA Microprep Plus Kit- Zymo Research
0097 Lavender Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-105171 Half Disarticulated Human Skeleton Axis Scientific
A-104270, 3-Part Life-Size Numbered Human Skull Axis Scientific
A-105759, Life-Size Human Skull With Removable Muscles Axis Scientific
A-105852 Classic Flexible Human Skeleton Axis Scientific
QIAamp DNA Blood Mini Kit (250) - 51106 - Qiagen
R1056- Quick-RNA Midiprep Kit 25 preps- Zymo Research
28506-QIAquick PCR & Gel Cleanup Kit (100)- Qiagen
A-105940, 22 Part Osteopathic Natural Bone Human Skull Axis Scientific
A-105170 Miniature Painted Human Skeleton Axis Scientific
A-105176 Disarticulated Half Child Skeleton Axis Scientific
A-104271, 3-Part Life-Size Painted Didactic Human Skull Axis Scientific
780801010-Short Heated Product Shelf Chamber- Labconco
0092 Blue Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
0094 Orange Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
RE801C-W- Rotary EvaporatorRE801C-W - Yamato
A-105867, Human Skull Model With Flexible Neck Axis Scientific
0090 Natural Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
0096 Well Microcentrifuge Tube Rack, Yellow, 5/Pk
19781-QIAfilter Mega-Giga Cartridges (5)- Qiagen
A11-000- SelectaLyte™ ISE Analyzer 510(k) Cleared- Awareness Technology
710201000-FreeZone 2.5 Liter -84C Benchtop Freeze Dryer- Labconco
3970325- 3' Purifier Filtered PCR Enclosure with UV Light, Protection Panel and Airflow Monitor- Labconco
28115-QIAquick Spin Columns (100)- Qiagen
3970403- 4' Purifier Vertical Clean Bench with Airflow Monitor- Labconco
711222010-FreeZone 12 Liter -84C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
3970223- 2' Purifier Vertical Clean Bench with Airflow Monitor- Labconco
700611030- FreeZone 6 Liter -50C Console Freeze Dryer- Labconco
0093F Fluorescent Green Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas 


