The Methylated-DNA IP Kit is designed for enrichment of 5mC-containing DNA from any pool of fragmented genomic DNA for use in genome-wide methylation analysis. It features a highly specific Anti-5-Methylcytosine Monoclonal Antibody for the immunoprecipitation of methylated DNA in only a few hours. This kit is capable of achieving over one hundred-fold enrichment of methylated DNA vs. non-methylated DNA. Recovered DNA is suitable for many downstream applications to analyze genome-wide DNA methylation including PCR, bisulfite treatment, whole-genome amplification, ultra-deep sequencing, and microarray. Control DNA and primers are included to monitor the success of the assay.
| Technical specifications | |||||||
| Applicable For | Immunoprecipitation of methylated DNA, PCR, sequencing, microarrays, etc. | ||||||
| Elution Volume | ≥ 15 µl | ||||||
| Input | 50 – 500 ng of DNA | ||||||
| Processing Time | 4 hours | ||||||
711222010-FreeZone 12 Liter -84C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
QIAamp DNA Blood Mini Kit (250) - 51106 - Qiagen
D4074- Quick-DNA Microprep Plus Kit- Zymo Research
AZI600-Azure 600-Azure BioSystems
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
711221010-FreeZone 12 Liter -84C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
28506-QIAquick PCR & Gel Cleanup Kit (100)- Qiagen
3970223- 2' Purifier Vertical Clean Bench with Airflow Monitor- Labconco
28506-QIAquick Spin Columns (100)- Qiagen
711211010-FreeZone 12 Liter -84C Console Freeze Dryer- Labconco
794801010-Stoppering Tray Dryer- Labconco
701222030- FreeZone 12 Liter -50C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
19781-QIAfilter Mega-Giga Cartridges (5)- Qiagen
A-105178, 3-Part Life-Size Human Skull With 8-Part Brain Axis Scientific
710202000-FreeZone 2.5 Liter -84C Benchtop Freeze Dryer- Labconco
0096 Well Microcentrifuge Tube Rack, Yellow, 5/Pk
R1056- Quick-RNA Midiprep Kit 25 preps- Zymo Research 


