The Methylated-DNA IP Kit is designed for enrichment of 5mC-containing DNA from any pool of fragmented genomic DNA for use in genome-wide methylation analysis. It features a highly specific Anti-5-Methylcytosine Monoclonal Antibody for the immunoprecipitation of methylated DNA in only a few hours. This kit is capable of achieving over one hundred-fold enrichment of methylated DNA vs. non-methylated DNA. Recovered DNA is suitable for many downstream applications to analyze genome-wide DNA methylation including PCR, bisulfite treatment, whole-genome amplification, ultra-deep sequencing, and microarray. Control DNA and primers are included to monitor the success of the assay.
| Technical specifications | |||||||
| Applicable For | Immunoprecipitation of methylated DNA, PCR, sequencing, microarrays, etc. | ||||||
| Elution Volume | ≥ 15 µl | ||||||
| Input | 50 – 500 ng of DNA | ||||||
| Processing Time | 4 hours | ||||||
700402030- FreeZone 4.5 Liter -50C Benchtop Freeze Dryer- Labconco
711211010-FreeZone 12 Liter -84C Console Freeze Dryer- Labconco
710611100-FreeZone 6 Liter -84C Console Freeze Dryer- Labconco
D4074- Quick-DNA Microprep Plus Kit- Zymo Research
710401000-FreeZone 4.5 Liter -84C Benchtop Freeze Dryer- Labconco
A-105170 Miniature Painted Human Skeleton Axis Scientific
700201030- FreeZone 2.5 Liter -50C Benchtop Freeze Dryer- Labconco
0091F Assorted Fluorescent Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
0095F Fluorescent Pink Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
0097 Lavender Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
700621130- FreeZone 6 Liter -50C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
794001010-FreeZone Triad Benchtop Freeze Dryer- Labconco
710202010-FreeZone 2.5 Liter -84C Benchtop Freeze Dryer- Labconco
710622010-FreeZone 6 Liter -84C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
0094 Orange Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
28506-QIAquick Spin Columns (100)- Qiagen
780701010-Bulk Tray Dryer- Labconco
711212110-FreeZone 12 Liter -84C Console Freeze Dryer- Labconco
700622030- FreeZone 6 Liter -50C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
0093F Fluorescent Green Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
D4075- Quick-DNA Midiprep Plus Kit- Zymo Research
710201010-FreeZone 2.5 Liter -84C Benchtop Freeze Dryer- Labconco
710611000-FreeZone 6 Liter -84C Console Freeze Dryer- Labconco
701812130-FreeZone 18 Liter -50C Console Freeze Dryer- Labconco
701811010-FreeZone 18 Liter -50C Console Freeze Dryer- Labconco
710402000-FreeZone 4.5 Liter -84C Benchtop Freeze Dryer- Labconco 



