The Methylated-DNA IP Kit is designed for enrichment of 5mC-containing DNA from any pool of fragmented genomic DNA for use in genome-wide methylation analysis. It features a highly specific Anti-5-Methylcytosine Monoclonal Antibody for the immunoprecipitation of methylated DNA in only a few hours. This kit is capable of achieving over one hundred-fold enrichment of methylated DNA vs. non-methylated DNA. Recovered DNA is suitable for many downstream applications to analyze genome-wide DNA methylation including PCR, bisulfite treatment, whole-genome amplification, ultra-deep sequencing, and microarray. Control DNA and primers are included to monitor the success of the assay.
| Technical specifications | |||||||
| Applicable For | Immunoprecipitation of methylated DNA, PCR, sequencing, microarrays, etc. | ||||||
| Elution Volume | ≥ 15 µl | ||||||
| Input | 50 – 500 ng of DNA | ||||||
| Processing Time | 4 hours | ||||||
R1101-1-10 - Feces Catcher 10 pack- Zymo Research
DKN-812C - Forced Convection Oven Programmable 300L 220V- Yamato
F123770-PIPETMAN Fixed F2, 2 µL-Gilson
R1002-YeaStar RNA Kit 40 Preps- Zymo Research
R2001- ZymoBIOMICS RNA Miniprep Kit 50 Preps- Zymo Research
F144056M-PIPETMAN G P20G, 2-20 µL, Metal Ejector-Gilson
R2024-Quick-RNA Plant Miniprep Kit 50 Preps- Zymo Research
F144071-PIPETMAN G Multichannel P12x20G, 2-20µL-Gilson
A-105173 Miniature Skull Axis Scientific
SF4200- Stat Fax®4200- Awareness Technology
F144058M-PIPETMAN G P200G, 20-200 µL, Metal Ejector-Gilson
F123607-PIPETMAN Fixed F5000, 5 mL-Gilson
F144801-PIPETMAN Classic P2-Gilson
F123789-PIPETMAN Fixed F400, 400 µL-Gilson
SAB 314e -Solis Semi-Micro and Analytical Balances- Adam Equipment
SF4500- Stat Fax®4500- Awareness Technology
RE301C-W - Rotary evaporator, Glass "C", Water BathRE301C-W - Yamato
F167300-PIPETMAN Classic Starter Kit, P20, P200, P1000-Gilson
SAB 225i -Solis Semi-Micro and Analytical Balances- Adam Equipment
H5000-20 Block, 35 x 2.0ml Benchmark Scientific
H5100-5DWMP- Block, 96x500µl plate, for MultiTherm Touch- Benchmark
PT 112 Plattforms Adam Equipment 



