The Methylated-DNA IP Kit is designed for enrichment of 5mC-containing DNA from any pool of fragmented genomic DNA for use in genome-wide methylation analysis. It features a highly specific Anti-5-Methylcytosine Monoclonal Antibody for the immunoprecipitation of methylated DNA in only a few hours. This kit is capable of achieving over one hundred-fold enrichment of methylated DNA vs. non-methylated DNA. Recovered DNA is suitable for many downstream applications to analyze genome-wide DNA methylation including PCR, bisulfite treatment, whole-genome amplification, ultra-deep sequencing, and microarray. Control DNA and primers are included to monitor the success of the assay.
| Technical specifications | |||||||
| Applicable For | Immunoprecipitation of methylated DNA, PCR, sequencing, microarrays, etc. | ||||||
| Elution Volume | ≥ 15 µl | ||||||
| Input | 50 – 500 ng of DNA | ||||||
| Processing Time | 4 hours | ||||||
A-105939, 22 Part Osteopathic Didactic Human Skull Axis Scientific
QIAamp DNA Blood Mini Kit (250) - 51106 - Qiagen
BCLO3015 Inox Protection Head Alliance Bio Expertise
DC4020- DRYCHILL™ 40 X 1.5/2.0ML- Benchmark
F144075-PIPETMAN G Multichannel P12x300G, 30-300µL-Gilson
GR146-1 -5X TBE Buffer 1LT- Hoefer
BCLO3003 Autoclavable Plastic Stick Grille - 90mm Box Alliance Bio Expertise
711212010-FreeZone 12 Liter -84C Console Freeze Dryer- Labconco
9120010-801 Micrometer reticule Labomed
3970223- 2' Purifier Vertical Clean Bench with Airflow Monitor- Labconco
F81051-PIPETMAN M P12X20M BT Connected-Gilson
BCLO3017 IQ/OQ protocol (French version) Alliance Bio Expertise
700201030- FreeZone 2.5 Liter -50C Benchtop Freeze Dryer- Labconco
F81043-PIPETMAN M P200M BT Connected-Gilson
M153- Buttockmate- Sakamoto
BGWR3005 Baggywel - Fraser Demi - 2 X 5l Alliance Bio Exterpise
S58L Premier Academic Kinesiology Skeleton, Painted And Labeled, Sacral Mount On Mobile Stand- Denoyer Geppert
3970302- 3' Purifier Filtered PCR Enclosure with UV Light and Protection Panel- Labconco
F81045-PIPETMAN M P1200M BT Connected-Gilson
BSH100-500 Block, 2 x 50ml Benchmark Scientific
M280 -Basic Lab Microscope- Unico
BGWR3001 Baggywel - Eau Peptonee Tamponnee - 2 X 5l Alliance Bio Exterpise
BSWCMB Quick-Flip Block Benchmark Scientific
701222030- FreeZone 12 Liter -50C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
BSH100-1213 Block, 15 x 12/13mm diameter tubes & vacutainers Benchmark Scientific
F123789-PIPETMAN Fixed F400, 400 µL-Gilson
F123603-PIPETMAN Classic P5000-Gilson
EPB 2103i -Equinox Precision Balances- Adam Equipment
0094F Fluorescent Orange Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
0093 Green Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
0096 Well Microcentrifuge Tube Rack, Yellow, 5/Pk
0092F Fluorescent Blue Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
0095F Fluorescent Pink Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
0091F Assorted Fluorescent Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
D4075- Quick-DNA Midiprep Plus Kit- Zymo Research
R1056- Quick-RNA Midiprep Kit 25 preps- Zymo Research 



